Method and apparatus for automatically determining the dilution curve of a solution, particularly the oxygen dissociation curve of blood or hemoglobin solutions

• US 4,097,921 A
• Filed: 11/24/1976
• Issued: 06/27/1978
• Est. Priority Date: 07/22/1975
• Status: Expired due to Term

• Current Assignee: Luigi Rossi
• Original Assignee: Luigi Rossi
• Inventors: Raffaele, Italo
• Primary Examiner(s): Wise, Edward J.
• Application Number:
  US05/744,825
• Time in Patent Office: 580 Days
• Field of Search: 235/151.35, 235/151.12, 23/230 B, 23/230 R, 23/254 E, 364/416
• US Class Current: 436/68
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3
• CPC Class Codes: Y10T 436/12 Condition responsive control
• CPC Class Codes: Y10T 436/163333 Organic [e.g., chemical warfare agents, insecticides, etc.]

Abstract

The invention relates to an apparatus for automatically determining the oxygen dissociation curve (ODC) of whole blood or of a hemoglobin solution. Blood or hemoglobin having a predetermined oxygen saturation level are admixed with deoxygenated blood or a hemoglobin solution and the oxygen partial pressure of the mixture, while being formed, is measured. The output of the P_O.sbsb.2 electrode, in the form of an electrical signal, is fed into a computer and two functions of the P_O.sbsb.2 (saturation and total oxygen) are calculated, giving the required curve by means of recording and/or displaying means. In this way, the ODC can be determined for whole blood under near physiological conditions of pH, hemoglobin concentration, hematocrit, temperature, P_CO.sbsb.2, and organic phosphates content.

Claims

1. A method for automatically determining the oxygen dissociation curve of whole blood or of hemoglobin solution, comprising the steps of continuously admixing a first sample of blood or hemoglobin solution containing oxygen at a known predetermined saturation level with a second sample of blood or hemoglobin solution at a different saturation level, continuously measuring the oxygen dissolved in the mixture, converting said measurement into an electrical signal, comparing said signal with reference values related to the said blood or hemoglobin solution having the predetermined saturation level, measuring the partial pressure of oxygen dissolved in the mixture and calculating the functions S = f (P_o) and Total O = f'"'"' (P_o) according to the equations:

##EQU5## in which;

V_o is the known volume of solution having said predetermined saturation level of oxygen,

S_o is the value of S calculated for the volume V_o,

C is the capacity of the solvent towards oxygen,

α

is the solubility coefficient of blood or hemoglobin solutions,

P_o is the partial pressure of oxygen in the known volume V_o,

V_a is the volume of the sample added and mixed with V_o at a time t,

P is the partial pressure of oxygen in the mixture at the time t,

S_a is the value of S for the mixture at the time t, the calculation being carried out in real time with respect to the mixing,

and

determining said dilution curve from the calculations.

2. A method according to claim 1, wherein the first sample is a sample of blood saturated with oxygen up to a predetermined level and the second sample is deoxygenated blood.

3. A method according to claim 2, wherein said step of admixing includes admixing the second sample of deoxygenated blood gradually with the entire first sample of oxygen saturated blood.

4. A method according to claim 3, wherein said step of determining includes plotting the values calculated by the computer on an X/Y recorder, whereby at the end of the mixing of the two samples, the oxygen dissociation curve of the blood under testing is obtained.

5. A method according to claim 4, wherein the step of plotting includes programming the display means of the recorder to hold the value of the oxygen partial pressure when S = 50 whereby the value of P₅₀ of the sample under analysis is automatically obtained.

6. A method according to claim 2, wherein said step of admixing includes mixing the two samples of blood, respectively saturated and deoxygenated, simultaneously at predetermined flow rates, and the oxygen partial pressure is measured on the mixture, as it is at a given time t.

7. An apparatus for automatically determining the oxygen dissociation curve of the whole blood or of hemoglobin solutions, comprising:

a thermostated mixing and measuring chamber,

first dosing means for feeding to the said chamber blood saturated with oxygen at a predetermined level;

second dosing means for feeding to the said chamber deoxygenated blood;

means for measuring the partial pressure of the oxygen on the mixture which is formed in said chamber and converting the measurement into an electrical signal;

an analogic computer, for receiving said signal and containing basic or reference data related to the oxygenated blood for calculating, in real time with respect to the mixing, the functions S = f (P_O.sbsb.2) and Total O₂ = f" (P_O.sbsb.2), and recording means for recording the calculated values of the two above said functions.

8. An apparatus according to claim 7, wherein said measuring means is an oxygen electrode.

9. An apparatus according to claim 7, wherein said first dosing means and said second dosing means are coincident, whereby said deoxygenated blood is fed to said chamber only after all the sample of oxygenated blood has been fed to the said chamber.