Glucose detection system free from fluoride-ion interference
First Claim
1. In a method for assaying for glucose in aqueous liquids by applying a sample of the aqueous liquid to a test element comprising a spreading layer and a reagent layer containing a reagent composition comprising a buffer, glucose oxidase, a substance having peroxidative activity and an indicator composition comprising 4-aminoantipyrine and a phenolic or naphtholic coupler such that glucose contained in the liquid sample is oxidized in the presence of said reagent composition to produce a colored product, the improvement comprising performing the said assay at a pH of between about 4.5 and 6.0 to reduce or eliminate interference with the assay due to fluoride ion which may be contained in the aqueous liquid sample.
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Abstract
An improved multilayer element for detecting glucose in samples of biological fluid which may contain with fluoride ion as a contaminant or preservative is described. The improved element comprises a buffered Trinder'"'"'s reagent, which reagent comprises glucose oxidase, peroxidase, 4-aminoantipyrine and a phenolic or naphtholic coupler, which is buffered to a pH between about 4.5 and about 6.0. Such a buffering reduces any interference which may be due to fluoride ion present in the sample under analysis.
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8 Claims
- 1. In a method for assaying for glucose in aqueous liquids by applying a sample of the aqueous liquid to a test element comprising a spreading layer and a reagent layer containing a reagent composition comprising a buffer, glucose oxidase, a substance having peroxidative activity and an indicator composition comprising 4-aminoantipyrine and a phenolic or naphtholic coupler such that glucose contained in the liquid sample is oxidized in the presence of said reagent composition to produce a colored product, the improvement comprising performing the said assay at a pH of between about 4.5 and 6.0 to reduce or eliminate interference with the assay due to fluoride ion which may be contained in the aqueous liquid sample.
- 5. In an integral element for the determination of glucose in aqueous liquids comprising a spreading layer and a reagent layer in fluid contact under conditions of use and containing as the glucose determining reagent composition Trinder'"'"'s reagent the improvement comprising the inclusion of a buffer which maintains the pH of the reagent composition between about 4.5 and 6.0 under conditions of use.
Specification