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Reverse sandwich immunoassay

  • US 4,098,876 A
  • Filed: 10/26/1976
  • Issued: 07/04/1978
  • Est. Priority Date: 10/26/1976
  • Status: Expired due to Term
First Claim
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1. A method of determining the presence or concentration of a polyvalent antigenic substance in a fluid sample which comprises the steps of:

  • (a) incubating the fluid sample with labeled antibodies to the substance under conditions sufficient to form a first labeled immunochemical complex;

    (b) incubating that complex with a composite comprising antibodies to the substance immobilized on a water insoluble carrier material, the incubation being under conditions sufficient to form a second labeled complex comprising the first complex complexed to the composite;

    (c) separating the second labeled complex from the incubation medium;

    (d) determining the amount of label associated with the second complex;

    (e) relating the determination of step (d) to a standard to determine the presence or concentration of the substance in the fluid.

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