Method for improving the activity of oxireductase enzymes embedded in filamentary structures

• US 4,100,029 A
• Filed: 07/09/1976
• Issued: 07/11/1978
• Est. Priority Date: 07/10/1975
• Status: Expired due to Term

• Current Assignee: ANIC SPA
• Original Assignee: SNAM Progetti SPA
• Inventors: Prosperi, Giulio, Morisi, Franco, Marconi, Walter, Giovenco, Silvia
• Primary Examiner(s): Shapiro, Lionel M.
• Application Number:
  US05/703,968
• Time in Patent Office: 732 Days
• Field of Search: 195/68, 195/63, 195/DIG. 11
• US Class Current: 435/182
• CPC Class Codes: C07J 1/0011 substituted in position 17 by a keto group
• CPC Class Codes: C07J 5/0053 not substituted in position 16
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12P 13/06 Alanine; Leucine; Isoleucine; Serine; Homoserine
• CPC Class Codes: C12P 33/06 Hydroxylating
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: D01F 1/02 Addition of substances to the spinning solution or to the melt addition of substances to viscose D01F2/08

Abstract

A method is disclosed for improving the activity of oxireductase enzymes, the improvement consisting in that an enzyme and a previously functionalized coenzyme are embedded in a filamentary structure, the coenzyme having previously been attached to a water-soluble, high-molecular-weight polymer.

Claims

1. A method for improving the activity of oxidoreductase enzymes that are formed into a filamentary structure which comprises a fibrous or filamentous structural base of a water-insoluble artifical synthetic polymeric material and an enzyme or enzymes therein englobed and subdivided and partially enclosed in separated alveoli, said method comprising the steps of forming solutions of a structural base of a water-insoluble artifical synthetic polymeric material an oxidoreductase enzyme and a coenzyme of nicotinamide-adenine-dinucleotide and a water soluble, high molecular weight polymer, forming an emulsion of said solutions and thereafter spinning said emulsion into said filamentary structure.

2. The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate and polyethyleneimine-nicotinamide-adenine-dinucleotide and the enzymes are lacticdehydrogenase and alanine-dehydrogenase.

3. The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate, formylpolyethyleneimine-nicotinamide-adenine-dinucleotide and the enzymes are lacticdehydrogenase and alanine-dehydrogenase.

4. The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate, polylysine-nicotinamide-adenine-dinucleotide and the enzymes are lacticdehydrogenase and alanine-dehydrogenase.

5. The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate polyethyleneimine-nicotinamide-adenine-dinucleotide, beta-hydroxysteroid dehydrogenase, alcohol dehydrogenase and aldehyde dehydrogenase.

6. The method of claim 1 wherein the filamentary structure is derived from a fibrous structural base which comprises cellulose triacetate, polyethyleneimine-nicotinamide-adenine-dinucleotide, beta-hydroxysteroid dehydrogenase and diaphorase.