Immobilized proteins

• US 4,102,746 A
• Filed: 08/16/1976
• Issued: 07/25/1978
• Est. Priority Date: 08/29/1975
• Status: Expired due to Term

• Current Assignee: FMC Corporation
• Original Assignee: Amerace Corporation (ABB Limited)
• Inventors: Goldberg, Bruce S.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US05/714,291
• Time in Patent Office: 708 Days
• Field of Search: 195/63, 195/68, 195/DIG. 11, 195/116, 264/49, 260/2.5 R, 260/112 R, 260/6, 210/65
• US Class Current: 435/96
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/14 Enzymes or microbial cells immobilised on or in an inorganic carrier
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier

Abstract

Proteins such as enzymes are immobilized on a microporous member comprising a binder or matrix and finely divided filler particles dispersed throughout the binder. The proteins are coupled to the filler particles, and the microporous member has a relatively large surface area and a large number of available protein coupling sites. Enzymes coupled to the microporous member have a relatively high reaction efficiency when used to act on a substrate.

Claims

1. An insoluble composite comprising a microporous member having at least a pair of opposed surfaces and a predetermined thickness, said microporous member comprising a polymeric resinous binder having finely divided filler particles dispersed throughout said binder and a network of substantially interconnected pores formed therein, said pores being formed within said resinous binder, between said filler particles and said resinous binder, and between neighboring filler particles, said dispersed filler particles being present in said microporous member in an amount by weight of at least about 25%, the size distribution of said pores varying non-uniformly across each of said surfaces and across said predetermined thickness through the range of about 0.01 micron to about 100 microns as determined porosimetrically by the Mercury Intrusion Method, and a proteinaceous substance bound to at least some of said filler particles dispersed throughout said binder, said microporous member being previous to the flow of a fluid through at least one of said surfaces wherein at least some of said filler particles to which said proteinaceous substance is bound is adapted to come into contact with such fluid.

2. The composite of claim 1 wherein said dispersed filler particles comprise silicious material.

3. The composite of claim 1 wherein said binder comprises polyvinyl chloride.

4. The composite of claim 1 wherein said binder comprises hard rubber.

5. The composite of claim 1 wherein said proteinaceous substance is a catalytically active enzyme.

6. The composite of claim 5 wherein said catalytically active enzyme is chemically bound to said at least some of said dispersed filler particles through an intermediate coupling agent.

7. The composite of claim 6 wherein said intermediate coupling agent is covalently bonded to said filler particles and said catalytically active enzyme is covalently bonded or crosslinked to said intermediate coupling agent.

8. The composite of claim 6 wherein said intermediate coupling agent is chemiadsorbed to the surface of said at least some of said dispersed filler particles and said catalytically active enzyme is covalently bonded or cross-linked to said intermediate coupling agent.

9. The composite of claim 5 wherein said enzyme is glucose oxidase.

10. The composite of claim 5 wherein said enzyme is aldose-1-epimerase.

11. The composite of claim 5 wherein said enzyme is α

-D-fructofuranosidase.

12. The composite of claim 5 wherein said enzyme is alcohol dehydrogenase.

13. The composite of claim 5 wherein said enzyme is glucose isomerase.

14. The composite of claim 1 wherein said finely divided filler particles comprise inorganic material.

15. The method of carrying out a chemical process comprising the steps of reacting a substrate by placing the substrate in contact with an insoluble microporous member having a proteinaceous substance which reacts with said substrate bonded thereto, and recovering a product of the reaction, said insoluble microporous member having at least a pair of opposed surfaces and a predetermined thickness, said microporous member comprising a polymeric resinous binder having finely divided filler particles dispersed throughout said binder and a network of substantially interconnected pores formed therein, said pores being formed within said resinous binder, between said filler particles and said resinous binder, and between neighboring filler particles, said dispersed filler particles being present in said microporous member in an amount by weight of at least about 25%, the size distribution of said pores varying non-uniformly across each of said surfaces and across said predetermined thickness through the range of about 0.01 micron to about 100 microns as determined porosimetrically by the Mercury Intrusion Method, and a proteinaceous substance bound to at least some of said filler particles dispersed throughout said binder, said microporous member being pervious to the flow of said substrate through at least one of said surfaces.

16. The method of claim 15 wherein said microporous member is in the form of a reactor core comprising a sheet having said at least pair of opposed surfaces extending substantially parallel to one another, and said step of reacting a substrate comprises passing said substrate through said sheet in a direction substantially normal to said parallel surfaces.

17. The method of claim 15 wherein said microporous member is in the form of a reactor core comprising a hollow tube having an inlet end and an outlet end, said step of enzymatically reacting a substrate comprises passing said substrate through said inlet end to contact the interior surface of said tube, and said product being recovered through said outlet end of said tube.

18. The method of claim 15 wherein said microporous member is in the form of a plurality of divided particles, and said step of enzymatically reacting a substrate comprises contacting said substrate with a plurality of said divided particles.

19. The method of claim 15 wherein said filler particles comprise inorganic material.