Method and apparatus for extravascular treatment of blood

• US 4,103,685 A
• Filed: 01/05/1976
• Issued: 08/01/1978
• Est. Priority Date: 01/05/1976
• Status: Expired due to Term

• Current Assignee: Robert Pritikin
• Original Assignee: John A. Awad, Paul J. Lupien, Sital Moorjani
• Inventors: Lupien, Paul J., Moorjani, Sital, Awad, John A.
• Primary Examiner(s): Truluck, Dalton L.
• Application Number:
  US05/646,567
• Time in Patent Office: 939 Days
• Field of Search: 128/214 C, 128/214 R, 128/214.2, 210/24 C, 210/28, 210/262, 210/DIG. 23, 23/258.5, 424/101, 260/112 B
• US Class Current: 604/5.03
• CPC Class Codes: A61M 1/3687 Chemical treatment A61M1/3675 takes precedence
• CPC Class Codes: A61M 2202/0057 retained by adsorption
• CPC Class Codes: A61M 2202/046 Low-density lipoprotein
• CPC Class Codes: Y10S 530/814 Cellulose or derivatives thereof

Abstract

There is provided a method and apparatus for the excorporeal treatment of blood of hyperlipemic anc hypercholesteremic patients whereby a portion of blood is drawn from such patients and treated with a divalent metal complex of a sulphated polysaccacharide coupled to a non-sulphated polysaccharide gel having its remaining sites blocked whereby a substantial amount of the lipoproteins present in the blood are bound to the gel and then filtering the blood before returning same to the patient.

Claims

1. A method for the extracorporeal treatment of the blood of a patient afflicted with hyperlipemia and/or hypercholesteremia to effect a reduction of the β

and pre-β

lipoproteins in said blood without substantially affecting the remaining constituents of the blood, which comprises;

(a) withdrawing a portion of blood from said patient,

(b) contacting said blood portion with a water-insoluble gel of a sulfated polysaccharide coupled to an activated non-sulfated polysaccharide having its remaining sites blocked, in the presence of calcium cation in a concentration of 0.02 to 0.04M, thereby selectively binding a substantial portion of the beta and pre-beta lipoproteins with the calcium complex gel,

(c) causing the thus treated blood portion to be filtered, thereby to separate the treated blood portion from the complex gel, whereby there is obtained the same blood portion wherein the initial amount of beta and pre-beta lipoproteins has been substantially reduced without affecting the remaining constituents of the blood, and

(d) returning the thus filtered blood portion to the patient.

2. The method of claim 1, wherein the sulfated polysaccharide is heparin sulfate.

3. The method of claim 1, wherein the sulfated polysaccharide is dextran sulfate.

4. The method of claim 1, wherein the non-sulfated polysaccharide is agarose.

5. In an apparatus for removing a substantial amount of β

0 and pre-β

lipoproteins from blood comprising recipient means for receiving therein blood to be treated;

means allowing the removal of treated blood from said recipient means; and

filter means disposed between said gel support and said means allowing treated blood removal, the improvement comprising a calcium metallic complex of sulfated polysaccharide coupled to an activated non-sulfated polysaccharide gel support wherein the remaining sites are blocked being contained in said recipient means whereby, when in the presence of said gel support, said blood is treated by the fixing of a substantial amount of β and

pre-β

lipoproteins to said support and said filter means having a porosity such as to prevent passage of said gel support therethrough as said treated blood is withdrawn from said recipient means.

6. An apparatus as defined in claim 5, wherein said gel support consists of agarose beads coated with heparin.

7. An apparatus for removing a substantial amount of β

and pre-β

lipoproteins from blood as claimed in claim 5, further comprising inlet means allowing blood to be treated to be received in said recipient means and outlet means allowing the removal of treated blood from said recipient means; and

said filter means being disposed between said gel support and said outlet means.

8. An apparatus for removing a substantial amount of β

and pre-β

lipoproteins from blood as claimed in claim 5, further comprising said recipient means having inlet means for receiving therein blood to be treated and outlet means for allowing the removal of treated blood from said recipient means;

said filter means being connected to said outlet means; and

further outlet means connected to said filter means for further discharging treated blood after having passed through said filter means.

9. An apparatus for removing a substantial amount of β

and pre-β

lipoproteins from blood as claimed in claim 5, further comprising outlet means connected to said recipient means;

said filter means being received in said recipient means and disposed between said gel support and said outlet means, said filter means having a porosity such as to prevent passage of said gel support therethrough as said treated blood passes through said outlet means.

10. An apparatus as defined in claim 9, wherein said recipient means includes inlet means allowing blood to be treated to be received therein;

further comprising syringe means for collecting blood from a patient'"'"'s vein;

said syringe means being adapted to be connected to said inlet means for discharging blood to be treated in said recipient means.

11. An apparatus as defined in claim 10, said outlet means including catheter means for returning treated blood to the patient;

said outlet means further comprising stopcock means connected to said catheter means whereby said syringe means may be connected to said stopcock means for the withdrawal of blood to be treated from said vein.

12. An apparatus for removing a substantial amount of β

and pre-β

lipoproteins from blood as claimed in claim 5, further comprising means for withdrawing blood from a patient'"'"'s vein; and

tube means for conducting blood collected from said vein to said recipient means;

said filter means being disposed between said gel support and said tube means;

said filter means having a porosity such as to prevent passage of said gel support therethrough as said treated blood is returned to said vein through said tube means.

13. An apparatus as defined in claim 5, wherein said filter means have a large surface area in comparison to the volume of blood to be treated.

14. An apparatus as defined in claim 13, wherein said filter means include successive layers of filtering surfaces having pores decreasing in size for the different layers with the smallest porosity being on the filtering surface closest to said means allowing the removal of treated blood.