Device for examining a plurality of microdoses of liquids

• US 4,106,911 A
• Filed: 06/29/1977
• Issued: 08/15/1978
• Est. Priority Date: 07/09/1976
• Status: Expired due to Term

• Current Assignee: Le Materiel Biomedical
• Original Assignee: Societe Francaise Pour Le Developpement De L'Automation In Biologie
• Inventors: Marcelli, Aline
• Primary Examiner(s): Serwin, R.E.
• Application Number:
  US05/811,298
• Time in Patent Office: 412 Days
• Field of Search: 23/230 R, 23/253 R, 23/259, 23/292, 195/127, 195/139, 141/284, 141/237, 141/238, 73/425.6
• US Class Current: 422/63
• CPC Class Codes: B01L 3/0227 Details of motor drive means B01L3/0231, B01L3/0234 take precedence
• CPC Class Codes: G01N 2035/1018 Detecting inhomogeneities, e.g. foam, bubbles, clots
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

A device for examining a plurality of microdoses of liquids by means of a urality of reagents, said device comprising in combination a dispenser for dispensing microdoses of reagents, comprising a plurality of vertical syringes adapted to be controlled simultaneously and arranged in a fixed pattern, a means for filling said syringes with reagents, provided with a plurality of receptacles containing said reagents and arranged in a fixed pattern corresponding to the pattern of said syringes, a receptacled assembly wherein a plurality of reaction receptacles are arranged in a fixed pattern likewise corresponding to the pattern of said syringes, and a dispenser for dispensing microdoses of liquids. The invention finds application in the testing of liquids, particularly in the field of cytology and immuno-haematology.

Claims

1. A device for examining a plurality of microdoses of liquids by means of a plurality of reagents, comprising in combination:

a frame;

a plurality of vertical syringes mounted on said frame and arranged in a fixed pattern, each of said syringes comprising a syringe body and a piston rod;

first means for imparting a relative vertical motion between the plurality of syringe bodies and the plurality of said piston rods, said first means being able to relatively move said syringe bodies and said piston rods between a near position and a remote position and vice versa as well as to a location between the near and remote positions, in order to draw up and dispense doses of liquids;

a support mounted on said frame and disposed below said plurality of vertical syringes;

second means for imparting a relative vertical motion between said plurality of syringe bodies and said support, said second means being able to relatively move said syringe bodies and said support between a near drawing up or dispensing position and a remote position;

first plate means, for filling said syringes with reagents, provided with a plurality of receptacles adapted to contain said reagents and arranged in a fixed pattern corresponding to the pattern of said syringes, said first plate means being adapted to be detachably supported by said support; and

second plate means provided with a plurality of reaction receptacles and arranged in a fixed pattern corresponding to the pattern of said syringes, said second plate means being adapted to be detachably supported by said support.

2. A device according to claim 1, wherein:

the syringe bodies are secured to the frame;

the piston rods are secured to a carriage mounted on said frame for a vertically reciprocal sliding motion; and

the support is vertically movable.

3. A device according to claim 2, wherein said carriage is moved under the action of a step-by-step electric motor actuating a vertical screw, said screw cooperating with a nut mounted on said carriage.

4. The device of claim 1, wherein the syringes of the microdose dispenser are composed of a plunger constituted by a rod provided with an annular groove in which is disposed an O-ring serving as a seal with the body of the syringe, which is formed by a tube, the needle itself being constituted by a concentric tube of smaller diameter, and the seal between the syringe body and the needle being ensured by means of a sealing material.

5. The device of claim 4, wherein the body and needle of the syringes are made of an electrically conducting material, whilst the seal between the syringe body and the needle is ensured by means of an electrically insulating material.

6. The device of claim 5, further comprising means for applying a potential difference between said bodies and said needles, a printed conductor adapted to measure the electrical potential of said needles and means for detecting, from the potential of said printed conductors, the variations in conductivity of the contents of the syringes near said needles.

7. The device of claim 1, wherein the means for filling the syringes with reagents also serves to package said reagent and comprises to this end a lid which hermetically covers it to close the receptacles.