Method and an automatic device for analyzing variations in the transparency of a sample

• US 4,109,159 A
• Filed: 05/24/1977
• Issued: 08/22/1978
• Est. Priority Date: 06/03/1976
• Status: Expired due to Term

• Current Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Original Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Inventors: Thenard, Jean, Roche, Pierre, Onillon, Michel
• Primary Examiner(s): Dahl, Lawrence J.
• Application Number:
  US05/799,935
• Time in Patent Office: 455 Days
• Field of Search: 73/64.1, 250/564, 250/573, 356/39, 356/201, 128/2 G
• US Class Current: 250/564
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)

Abstract

The method consists in generating an electrical signal B which is representative of the time-dependent variations in transparency, in generating a pulse at the initial instant of measurement, in carrying out an initial measurement of the slope of the curve at the beginning of the portion which has a steep slope, in comparing at predetermined instants the measured value of the initial slope with the slope of the curve which is representative of the signal B at these instants, in generating a second pulse when the measured slope becomes significantly different from the initial slope, and in measuring the time interval which elapses between the first and second pulses.

Claims

1. A method for analyzing the time of useful variaton in transparency of a sample in which the end of the useful period of said variations is characterized by a variation in slope of the curve which is representative of said transparency as a function of time, wherein said method consists in generating an electrical signal B which is representative of the time-dependent variations of said transparency, in generating a pulse at the initial instant of measurement, in carrying out an initial measurement of the slope of the curve at the beginning of the portion which has a steep slope, in comparing at predetermined instants the measured value of said initial slope with the slope of the curve which is representative of the signal B at said instants, in generating a second pulse when the measured slope becomes significantly different from the initial slope, and in measuring the time interval which elapses between the first and second pulses.

2. A method according to claim 1, wherein said method consists in generating a second variable signal A, in generating a first pulse at the initial instant of measurement, in carrying out a measurement of the slope of the representative curve at the beginning of the high-slope portion of said curve by increasing the signal A by a value N during a time interval t until the signal B becomes equal to the signal A, the time interval t being stored in memory, in comparing said slope defined by the ratio N/t with the slope of the signal B, in generating a second pulse when the slope of the signal B becomes smaller than the ratio N/t and in measuring the time interval which elapses between the first and the second pulse.

3. A method according to claim 2, wherein the signals A and B are generated in digital form and the value N is a number.

4. A method according to claim 3, wherein the time comparison is carried out by comparing the numerical value of the signal B with the numerical value of the signal A which is incremented by the number N at each time interval t and wherein a second pulse is emitted when the value of the signal A becomes higher than that of the signal B.

5. A device for analyzing time-dependent variations in transparency of a sample in which said variations have a high-slope region followed by a low-slope region, wherein said device comprises means for generating a first signal B which is representative of the time-dependent variations of said transparency, means for generating a second signal A which is variable in time from an initial instant t₀, means for generating a first pulse at the initial instant t₀, means for generating the signal A, said means being provided with means for making said signal representative of the initial slope of the signal B at the beginning of its high-slope region, means for periodically comparing said slope with the slope of the signal B, means for generating a second pulse when the slope of the signal B becomes higher than the initial slope measured, and means for measuring the time interval which elapses between the first and the second pulses.

6. A device according to claim 5, wherein said transparency increases as a function of time, wherein the means for generating the signal B comprise means for periodically illuminating said simple, means for collecting the light signal which has passed through said simple, and means for converting said light signal to an electrical signal which is proportional in time to the amplitude of said collected light signal.

7. A device according to claim 5, wherein the transparency of the sample decreases as a function of time, and wherein said device comprises means for generating the signal B including means for periodically illuminating said sample, means for collecting the light signal which has passed through said sample and for converting said light signal to an electrical signal which is proportional in time to the amplitude of said light signal, and means for complementing said signal B to a value which is higher than the maximum value of said signal B.

8. A device according to claim 5, wherein the means for illuminating and collecting the light signal are constituted by an electroluminescent diode which is supplied during finite periods of time, said diode being placed on one side of the sample, and by a phototransistor placed on the other side of the sample and capable of collecting the light signal which has passed through said sample.

9. A device according to claim 5, wherein the means for making the signal A representative of the slope of the signal B comprise means for defining an instant T₁ corresponding to the beginning of the high-slope region, means for giving the signal A at said instant T₁ a value equal to the value B₁ of the signal at said instant and for adding a quantity N to said value B₁ in order to ensure that the signal A is of higher value than the signal B at said instant, means for detecting the instant T₂ at which the signal B becomes equal to the value B₁ + N, and means for measuring the time interval t which elapses between the instants T₁ and T₂. the ratio N/t being intended to give the value of said slope.

10. A device according to claim 9, wherein the means for defining the instant T₁ consist of means for giving the signal A at the initial instant t₀ the value B₀ which the signal B has at said instant t₀ increased by the value N, means for detecting the instant T₁ at which the signal B assumes the value B₀ + N, means for giving the value B₀ + 2N to the signal A at said instant and means for detecting the instant at which the signal B becomes equal to B₀ + 2N, said instant being the instant T₁.

11. A device according to claim 5, wherein the means for comparing the slope of the signal B at each instant with the slope of the signal B at the beginning of the high-slope zone comprise means for giving the signal A at an instant T₃ which is slightly later in time than the instant T₂ a value A₃ which is slightly lower than the value of the signal B at said instant, means for giving a stairstep wave form to the signal A starting from an instant T₃ by adding the value N to the signal at each instant T₃ + nt, and means for comparing the value of the signal A with the value of the signal B and for generating said second pulse when the signal A becomes higher in value than the signal B.