Analytical element for clinical analysis
First Claim
1. An analytical element for the assay of an aqueous liquid sample, said element comprising(a) a porous layer structure in which said assay is carried out, said structure being one or more layer compositions selected from the group consisting of porous paper or fabric compostions, permeable polymer film compositions, particle-containing compositions having interconnected open spaces between said particles, and mixtures of the foregoing compositions;
- said porous layer structure comprising(i) a porous layer bearing a substantially planar sample receiving surface to receive said liquid sample from an external source, said porous layer having a layer composition selected from the group defined in (a) hereinabove.(ii) at least one dry reagent material for use in said assay, said reagent material being contained in said porous layer or in a separate porous reagent layer having a layer composition selected from the group set forth in (a) hereinabove, and(iii) at least one reaction zone comprising a portion of said porous layer, or said porous reagent layer, or a separate porous layer having a composition selected from the group set forth in (a) hereinabove, at least one reaction employed in said assay being carried out in said reaction zone, each of said porous layer, said porous reagent layer, and said reaction zone being in fluid contact with one another; and
(b) a rupturable pod-like member containing a liquid reagent for said assay, said member being associated with said reaction zone whereby said liquid reagent, upon rupture of said member, is released into said reaction zone.
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Accused Products
Abstract
An analytical element for use in the clinical assay of an analyte contained in a biological liquid sample which is applied from an external source to a substantially planar sample receiving suface of the element. The analytical element comprises a porous layer bearing the aforementioned sample receiving surface; at least one dry reagent material for use in the assay; and one or more associated rupturable pod-like member(s) containing a liquid reagent for the assay, such liquid reagent, upon rupture of the pod-like member(s), being released into the element.
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Citations
10 Claims
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1. An analytical element for the assay of an aqueous liquid sample, said element comprising
(a) a porous layer structure in which said assay is carried out, said structure being one or more layer compositions selected from the group consisting of porous paper or fabric compostions, permeable polymer film compositions, particle-containing compositions having interconnected open spaces between said particles, and mixtures of the foregoing compositions; - said porous layer structure comprising
(i) a porous layer bearing a substantially planar sample receiving surface to receive said liquid sample from an external source, said porous layer having a layer composition selected from the group defined in (a) hereinabove. (ii) at least one dry reagent material for use in said assay, said reagent material being contained in said porous layer or in a separate porous reagent layer having a layer composition selected from the group set forth in (a) hereinabove, and (iii) at least one reaction zone comprising a portion of said porous layer, or said porous reagent layer, or a separate porous layer having a composition selected from the group set forth in (a) hereinabove, at least one reaction employed in said assay being carried out in said reaction zone, each of said porous layer, said porous reagent layer, and said reaction zone being in fluid contact with one another; and (b) a rupturable pod-like member containing a liquid reagent for said assay, said member being associated with said reaction zone whereby said liquid reagent, upon rupture of said member, is released into said reaction zone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- said porous layer structure comprising
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10. A method for the assay of an aqueous liquid sample comprising:
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(1) applying said liquid sample to an analytical element comprising a dry reagent material and a liquid reagent, both of which interact in said assay to produce a detectable change in said element corresponding to the presence of analyte, said element comprising (a) a porous layer structure in which said assay is carried out, said structure being one or more layer compositions selected from the group consisting of porous paper or fabric compositions, permeable polymer film compositions, particle-containing compositions having interconnected open spaces between said particles, and mixtures of the foregoing compositions;
said porous layer structure comprising(i) a porous layer bearing a substantially planar sample receiving surface to receive said liquid sample from an external source, said porous layer having a layer composition selected from the group defined in (a) hereinabove, (ii) at least one dry reagent material for use in said assay, said reagent material being contained in said porous layer or in a separate porous reagent layer having a layer composition selected from the group set forth in (a) hereinabove, and (iii) at least one reaction zone comprising a portion of said porous layer, or said porous reagent layer, or a separate porous layer having a composition selected from the group set forth in (a) hereinabove, at least one reaction employed in said assay being carried out in said reaction zone, each of said porous layer, said porous reagent layer, and said reaction zone being in fluid contact with one another; and (b) a rupturable pod-like member containing a liquid reagent for said assay, said member being associated with said reaction zone whereby said liquid reagent, upon rupture of said member, is released into said reaction zone; (2) rupturing said pod-like member of said element to release said liquid reagent into said reaction zone of said element; and (3) detecting said change produced in said element.
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Specification