Immunological testing devices and methods

• US 4,111,754 A
• Filed: 11/29/1976
• Issued: 09/05/1978
• Est. Priority Date: 11/29/1976
• Status: Expired due to Term

• Current Assignee: Hydow Park
• Original Assignee: Hydow Park
• Inventors: Park, Hydow
• Primary Examiner(s): Tanenholtz, Alvin E.
• Assistant Examiner(s): Fan, C. A.
• Application Number:
  US05/745,541
• Time in Patent Office: 645 Days
• Field of Search: 195/127, 195/103.5 R, 195/103.5 A, 23/253 R, 424/12
• US Class Current: 435/288.5
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543

Abstract

A self-supporting plastic structure having a plurality of through-passages, each passage being of cross-sectional shape and area such that walls thereof provide a large amount of surface area relative to a substantially small amount of space cross-sectional area, each through passage having an upper funnel portion for receiving liquid, and a small outlet aperture of a smallness sufficient to effect a retention of liquid drop(s) within space of the through-passage by capillary action in the absence of additionally added fluid which by weight thereof forces liquid from the outlet aperture, and a separate washer structure seatable above the plastic structure and having a main liquid-containable vessel defined with a plurality of open outlets in the base thereof aligned for feeding contained liquid into separate ones of the through-passages simultaneously for the concurrent washing of each and all and/or for concurrent adding of further reagent or the like simultaneously concurrently to each and all.

Claims

1. An immunological testing device comprising:

a plastic structure having upper and lower open ends, having a through-passage of predetermined small cross-sectional area sufficiently small to retain liquid by capillary action within space thereof at at-least substantially an intermediate portion of a length thereof, and the plastic structure being elongated along a longitudinal axis of the through-passage, the ratio of the wall surface area of said through-passage being larger than the cross-sectional area of said intermediate portion, and in which said plastic structure has formed therein an enlarged funnel structure in continuous flow-relationship with the upper end of the intermediate portion of the through-passage of said predetermined small cross-sectional area.

2. An immunological testing device of claim 1, in which said plastic structure has only a single through-passage.

3. An immunological testing device of claim 1, in which said plastic structure includes a plurality of said through-passages arranged in substantially parallel-flow relationship;

and tubular collector-structure forming a common collecting space at lower-end outlets of said plurality.

4. An immunological testing device of claim 1, including a washing structure having a liquid containing structure with a plurality of spaced-apart funnel-outlets, and in which said plastic structure includes a plurality of said through-passages and of said enlarged funnel structures, and washing structure being seatable onto an upper portion of said enlarged funnel structures with each one respectively of the enlarged funnel structures positioned for receiving liquid flow from at least one of said spaced-apart funnel-outlets, the spaced-apart funnel-outlets thereby feeding washing liquid to separate ones of said enlarged funnel structures whereby through-passages associated with respective ones of each enlarged funnel structure are concurrently washable so as to be devoid of contamination between separate ones of the enlarged funnel structures.

5. An immunological testing device of claim 1, in which said plastic structure includes a plurality of said through-passages arranged in substantially parallel-flow relationship and in which through-flow spaces of said plurality of said through-passages are in flow-communication with one-another;

and including a washing structure having a liquid containing structure with a plurality of spaced-apart funnel-outlets, said washing structure being seatable onto an upper portion of structure of a plurality of said enlarged funnel structure with each one respectively of the enlarged funnel structures positioned for receiving liquid flow from at least one of said spaced-apart funnel-outlets, the spaced-apart funnel-outlets thereby feeding washing liquid to separate ones of said enlarged funnel structures whereby through-passages associated with respective ones of each enlarged funnel structure are concurrently washable so as to be devoid of contamination between separate ones of the enlarged funnel structures.

6. An immunological testing device of claim 1 including a plurality of said enlarged funnel structure and pluralities of said through-passages, through-passages of different enlarged funnel structures being in segregated non-flow relationship to one-another.

7. An immunological testing device of claim 5, including means for detaching individual sets of an enlarged funnel structure and associated plurality of through-passages thereof, from other ones of enlarged funnel structures and associated through-passages thereof.

8. An immunological testing device of claim 7, in which said means for detaching comprises scored structure adapted for ease of severing structures from one-another.

9. An immunological testing device of claim 3, in which said tubular collector structure has a lower outlet aperture of predetermined small dimensional cross-section sufficiently small to effect retaining a drop of liquid within said common collecting space when devoid of liquid weight of additional liquid above said intermediate portion within said enlarged funnel structure.

10. An immological testing device of claim 3, in which spaces of the plurality of said through-passages are in common communication along longitudinal lengths thereof within a space formed centrally therebetween and interconnecting the through-passages of the plurality of said through-passages.

11. An immunological testing device of claim 10, in which said tubular collector structure has a lower outlet aperture of predetermined small dimensional cross-section sufficiently small to effect retaining a drop of liquid within said common collecting space when devoid of liquid weight of additional liquid above said intermediate portion within said enlarged funnel structure.