Apparatus for preparative electrophoresis

• US 4,111,785 A
• Filed: 05/16/1977
• Issued: 09/05/1978
• Est. Priority Date: 05/18/1976
• Status: Expired due to Term

• Current Assignee: Willem Gerrit Roskam
• Original Assignee: Willem Gerrit Roskam
• Inventors: Roskam, Willem Gerrit
• Primary Examiner(s): Prescott, Arthur C.
• Application Number:
  US05/797,286
• Time in Patent Office: 477 Days
• Field of Search: 204/180 R, 204/180 G, 204/299, 204/301
• US Class Current: 204/615
• CPC Class Codes: C25B 7/00 Electrophoretic production of compounds or non-metals separation or purification of peptides, e.g. of proteins, by electrophoresis C07K1/26
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones

Abstract

Electrophoresis apparatus comprises a tubular member for receiving a column of gel, and a plate-form body of solid but porous material, such as glass fit, defining an elution chamber at one end of the tubular member. An inlet duct delivers elution buffer into the plate-form body by way of one of the main faces thereof.

Claims

1. Electrophoresis apparatus, comprising a tubular member having first and second ends for receiving a column of gel, means defining an elution chamber at the first end of the tubular member, primary inlet duct means for passing buffer solution into the elution chamber, and outlet duct means for discharging buffer solution from the elution chamber and so disposed with respect to the primary inlet duct means that buffer solution passes from the primary inlet duct means to the outlet duct means substantially perpendicular to the central axis of the tubular member, the chamber-defining means comprising a plate-form body of solid but porous material having two main surfaces extending substantially perpendicular to the central axis of said tubular member, and the apparatus also comprising auxiliary inlet duct means for passing an auxiliary flow of buffer solution into the elution chamber through that main surface of the plate-form body which is further from the second end of the tubular member, and a layer of porous material between the elution chamber and the auxiliary inlet duct means, the porous material of said layer having a higher flow resistance to buffer solution than the porous material of the plate-form body.

2. Apparatus as claimed in claim 1, wherein the plate-form body is a glass frit.

3. Apparatus as claimed in claim 2, wherein the layer of porous material is a glass frit.

4. Apparatus as claimed in claim 3, wherein the plate-form body and the layer of porous material are sintered together.

5. Apparatus as claimed in claim 1, wherein the plate-form body is sealed to said tubular member by means of a layer of vulcanized elastic material adhered to the plate-form body.

6. Apparatus as claimed in claim 1, wherein the plate-form body is sealed to said auxiliary inlet duct means by means of a layer of vulcanized elastic material adhered to the plate-form body.

7. Apparatus as claimed in claim 6, wherein the plate-form body and the auxiliary inlet duct means are fitted in a cylindrical housing member and the layer of vulcanized elastic material establishes a seal between the plate-form body and the housing member.

16. Apparatus as claimed in claim 1, wherein the tubular member is of circular internal cross section.

17. Apparatus as claimed in claim 6, wherein the elastic material is silicone rubber.

18. Apparatus as claimed in claim 7, wherein the elastic material is silicone rubber.

8. Electrophoresis apparatus, comprising a tubular member having first and second ends for receiving a column of gel, and means defining an elution chamber at the first end of the tubular member, the chamber-defining means comprising a plate-form body of solid but porous material having two main surfaces, one main surface of the plate-form body being directed towards the tubular member, and the apparatus also comprising duct means for delivering buffer solution into the elution chamber by way of the other main surface of the plate-form body, and a layer of porous material between the elution chamber and the duct means, the porous material of said layer having a higher flow resistance to buffer solution than the porous material of the plate-form body.

9. Apparatus as claimed in claim 8, wherein the plate-form body is a glass frit.

10. Apparatus as claimed in claim 9, wherein the layer of porous material is a glass frit.

11. Apparatus as claimed in claim 10, wherein the plate-form body and the layer of porous material are sintered together.

12. Apparatus as claimed in claim 9, wherein the plate-form body is sealed to said tubular member by means of a layer of vulcanized elastic material adhered to the plate-form body.

13. Apparatus as claimed in claim 11, wherein the plate-form body and the duct means are fitted in a cylindrical housing member and the layer of vulcanized elastic material establishes a seal between the plate-form body and the housing member.

15. A method as claimed in claim 13, wherein the plate-form body has two main surfaces, one of which is brought into contact with said one end of the tubular member and the other of which is brought into contact with duct means for delivering buffer solution into the plate-form body, and the vulcanized elastic material establishes a seal between the plate-form body and the duct means.

19. Apparatus as claimed in claim 8, wherein the housing member is of circular internal cross-section.

20. Apparatus as claimed in claim 9, wherein the tubular member is of circular internal cross-section.

21. Apparatus as claimed in claim 11, wherein the elastic material is silicone rubber.

22. Apparatus as claimed in claim 12, wherein the housing member is of circular internal cross-section.

23. A method as claimed in claim 13, wherein the elastic material is silicone rubber.

14. A method of constructing electrophoresis apparatus, wherein a layer of elastic material is applied to a plate-form body of solid but porous material, the elastic material is vulcanized, and the plate-form body is brought into contact with one end of a tubular member, the vulcanized elastic material establishing a seal between the plate-form body and the tubular member.