Kinetic method for determination of glucose concentrations with glucose dehydrogenase

• US 4,120,755 A
• Filed: 04/28/1977
• Issued: 10/17/1978
• Est. Priority Date: 04/28/1977
• Status: Expired due to Term

• Current Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Original Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Inventors: Tung, Ker-Kong, Pierre, Kenneth J.
• Primary Examiner(s): Shapiro, Lionel M.
• Application Number:
  US05/791,825
• Time in Patent Office: 537 Days
• Field of Search: 195/103.5 C, 195/103.5 R
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/54 involving glucose or galactose

Abstract

The range of linearity of the kinetic method for the determination of glucose concentrations using a reagent of the type comprising a buffer, a pyridine coenzyme, and glucose dehydrogenase, is significantly increased when the buffer employed in the reagent has a pH of from about 7.9 to about 9.0.

Claims

1. A kinetic glucose reagent of the type comprising a buffer, a pyridine coenzyme, and glucose dehydrogenase, characterized in that said buffer has a pH of from about 7.9 to about 9.0.

2. The kinetic glucose reagent of claim 1 wherein said buffer has a pH of from about 8.0 to about 8.5.

3. The kinetic glucose reagent of claim 2 wherin said buffer is selected from a group consisting of triethanolamine sodium 5:

5-diethylbarbiturate-HCl, tris(hydroxymethyl) aminomethane, diethanolamine, and phosphate buffers.

4. The kinetic glucose reagent of claim 2 wherein said buffer is a phosphate buffer selected from a group consisting of potassium phosphate and sodium phosphate.

5. The kinetic glucose reagent of claim 2 wherein said buffer has a pH of about 8.2.

6. The kinetic glucose reagent of claim 5 wherein said buffer is selected from a group consisting of triethanolamine sodium 5:

5-diethylbarbiturate-HCl, tris(hydroxymethyl) aminomethane, diethanolamine, and phosphate buffers.

7. The kinetic glucose reagent of claim 5 wherein said buffer is a phosphate buffer selected from a group consisting of potassium phosphate and sodium phosphate.

8. The kinetic glucose reagent of claim 1 wherein said buffer is selected from a group consisting of triethanolamine sodium 5:

5-diethylbarbiturate-HCl, tris(hydroxymethyl) aminomethane, diethanolamine, and phosphate buffers.

9. The kinetic glucose reagent of claim 1 wherein said buffer is a phosphate buffer selected from a group consisting of potassium phosphate and sodium phosphate.

10. The kinetic glucose reagent of claim 1 comprising per liter of reagent:

(a) from about 0.1 to about 1 mole of said buffer;

(b) from about 0.1 to about 1 mole of an alkali chloride;

(c) from about 0 to about 0.02 mole of EDTA;

(d) from about 0.001 to about 0.025 mole of said pyridine coenzyme; and

(e) from about 100 to about 3000 International Units of glucose dehydrogenase.

11. The kinetic glucose reagent of claim 10 comprising per liter of reagent:

(a) about 0.5 mole of said buffer;

(b) about 0.5 mole of said alkali chloride;

(c) about 0.005 mole of EDTA;

(d) about 0.0027 mole of said pyridine coenzyme; and

(e) about 1600 International Units of glucose dehydrogenase.

12. An improved kinetic assay of the type employing a kinetic glucose reagent comprising a buffer, a pyridine coenzyme and glucose dehydrogenase, wherein the improvement comprises conducting said assay at a pH of from about 7.9 to about 9.0.

13. The kinetic assay of claim 12 wherein said assay is conducted at a pH at from about 8.0 to about 8.5.

14. The kinetic assay of claim 13 wherein said assay is conducted at a pH of about 8.2.