Method and apparatus for simultaneously recording reaction times

• US 4,125,327 A
• Filed: 11/01/1976
• Issued: 11/14/1978
• Est. Priority Date: 11/17/1975
• Status: Expired due to Term

• Current Assignee: Arturus Capital Ltd.
• Original Assignee: Gradient, Inc. (Ag Leader Technology Incorporated)
• Inventors: Margolis, Joel
• Primary Examiner(s): McGraw, Vincent P.
• Assistant Examiner(s): Bovernick, Rodney
• Application Number:
  US05/737,523
• Time in Patent Office: 743 Days
• Field of Search: 356/201, 356/39, 356/180, 356/208, 356/184, 356/197, 259/51, 259/52, 259/112, 259/70, 259/71, 23/230 B, 23/253 R
• US Class Current: 356/39
• CPC Class Codes: B01F 35/213 of the properties of the mixtures, e.g. temperature, density or colour
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00

Abstract

An apparatus and method of determining the end point of a chemical reaction producing an optical change in a plurality of samples is disclosed. The invention is particularly suitable for the simultaneous determination of the coagulation time for multiple samples of blood and like materials.

Claims

1. Apparatus for simultaneously determining the completion time, of chemical reactions producing an optical change, in a plurality of chemical samples each of which is contained in a transparent container and includes a suspension containing fibrin and an added particulate substance to which fibrin is capable of adhering, said apparatus comprising retention means to locate the plurality of containers with each container interposed in a light path between a corresponding light source and a corresponding light sensor, and agitator insertable within said containers and movable to agitate said samples, a timer connected with said light sensors and operable by the outputs thereof, and means for switching on the timer, the latter means being operated with the commencement of the reaction in the containers, and means for stopping the timer constituted by the provision wherein, said particulate substance is so constituted that fibrin mesh formed by the samples during the chemical reaction traps the particles of said suspension and is swept away during agitation of the samples such that the timer is stopped by an increase of light to the detector.

2. 2. The apparatus as claimed in claim 1 wherein each said container has an open top, said retention means retains said containers upright and positioned in a row, and said agitator is transparent and comprises a comb having a base and a plurality of spaced parallel fingers, each of said fingers being insertable into a corresponding one of said containers via its open top.

3. The apparatus as claimed in claim 2 wherein the base of said comb has a slot therethrough transverse to said fingers and a pin is slidably positioned in said slot, said pin being substantially parallel to and displaced from a shaft, whereby rotation of said shaft produces longtudinal reciprocation of said fingers.

4. The apparatus as claimed in claim 3 including control means to maintain said samples at a constant temperature while said containers are located in said retention means.

5. The apparatus as claimed in claim 4 wherein said control means comprises a heated metal block, said retention means comprises an array of container receiving recesses in said block, said light sources are positioned to one side of said block, said light sensors are positioned on the other side of said block, and each said light path comprises a bore extending between said sides of said block and through the corresponding container receiving recess.

6. The apparatus as claimed in claim 5 including preheating means comprising a metal body in thermal communication with said heated block and including a like number of container receiving recesses.

12. The apparatus as claimed in claim 3 wherein said pin is removable from said slot to enable separation of said comb from said shaft and removable of the comb from said containers.

7. A method of simultaneously determining the completion time, of a chemical reaction producing an optical change, in a plurality of chemical samples comprising a suspension containing fibrin, said method comprising the steps of adding to each sample a particulate substance to which fibrin is capable of adhering, placing each said sample in a transparent container, initiating said reaction and simultaneously switching-on a timing means, agitating said samples within said containers so that a fibrin mesh formed in the samples traps the particles of the suspension and the mesh is swept away during the agitation of the samples to cause the samples to be suddenly and rapidly rendered transparent, and switching-off said timing means when the samples are rendered transparent.

8. The method as claimed in claim 7 wherein each said container is placed in a corresponding light path between a corresponding light source and a corresponding light sensor, and said timing means is switched off in response to the outputs of said sensors.

9. The method as claimed in claim 8 wherein said samples are maintained at a pre-determined temperature while positioned in said light paths.

10. The method as claimed in claim 7 wherein said particles are kaolin, cellulose or diatomaceous earth.

11. The method as claimed in claim 10 wherein said containers have open tops and each said sample is agitated by longitudinal reciprocation of an elongated finger extending through the open top of the container holding said sample.