Biomass growth restriction in a packed bed reactor

• US 4,127,447 A
• Filed: 05/03/1976
• Issued: 11/28/1978
• Est. Priority Date: 05/03/1976
• Status: Expired due to Term

• Current Assignee: United States Of America As Represented By The Department Of Energy
• Original Assignee: United States Department of Energy (Government of the United States of America)
• Inventors: Griffith, William L., Compere, Alicia L.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US05/682,801
• Time in Patent Office: 939 Days
• Field of Search: 195/59, 195/27, 195/31, 195/2, 195/115, 195/116, 195/117, 195/110, 195/111, 210/2, 210/11, 210/17
• US Class Current: 435/162
• CPC Class Codes: C02F 3/2806 Anaerobic processes using solid supports for microorganisms
• CPC Class Codes: C02F 3/34 characterised by the microorganisms used
• CPC Class Codes: C12N 1/105 Protozoal isolates
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12P 5/023 Methane
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: C12R 2001/90 Protozoa ; Processes using protozoa
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel
• CPC Class Codes: Y10S 435/801 Anerobic cultivation
• CPC Class Codes: Y10S 435/813 Continuous fermentation
• CPC Class Codes: Y10S 435/947 using protozoa

Abstract

When carrying out continuous biologically catalyzed reactions with anaerobic microorganisms attached to a support in an upflow packed bed column, growth of the microorganisms is restricted to prevent the microorganisms from plugging the column by limiting the availability of an essential nutrient and/or by the presence of predatory protozoa which consume the anaerobic microorganisms. A membrane disruptive detergent may be provided in the column to lyse dead microorganisms to make them available as nutrients for live microorganisms.

Claims

1. A process for carrying out a biologically catalyzed reaction comprising the steps of:

introducing an influent solution containing reactants into the lower section of an upflow packed bed column packed with a support material for microorganisms, said support material having attached thereto anaerobic microorganisms for catalyzing the reaction of said reactants;

introducing with said influent a membrane disruptive detergent to lyse dead microorganisms from said support and to make available the essential nutrient of said dead microorganisms to the remaining live microoganisms;

allowing said reactants to react under the influence of said anaerobic microorganisms; and

simultaneously with said steps of introducing and allowing restricting the presence of an essential growth nutrient within said influent solution to cause the population of said anaerobic microorganism to remain substantially constant and to prevent said anaerobic microoganisms from plugging said packed column.

2. The process according to claim 1 wherein said microorganisms convert BOD of said influent solution to methane.

3. The process according to claim 1 wherein said influent contains glucose as a reactant wherein said glucose is converted to ethanol by said microorganisms and wherein said microoganisms are yeast and the presence of nitrogen is restricted to cause the population of said microorganisms to remain substantially constant.

4. The process according to claim 1 wherein said support material has a hydrophilic charged surface having anaerobic microorganisms attached to said hydrophilic charged surface.

5. The process according to claim 1 wherein said support material has a polymeric surface having anaerobic microorganisms attached to said polymeric surface.

6. The process according to claim 1 wherein said support material has a crosslinked polymeric surface having anaerobic microorganisms attached to said crosslinked polymeric surface.

7. The process according to claim 6 wherein said influent contains glucose as a reactant wherein said glucose is converted to ethanol by said microorganisms and wherein said microorganisms are yeast and the presence of nitrogen is restricted to cause the population of said microorganisms to remain substantially constant.

8. A process for carrying out a biologically catalyzed reaction comprising the steps of:

introducing an influent solution containing reactants into the lower section of an upflow packed bed column packed with a support material for microorganisms, said support material having attached thereto anaerobic microorganisms for catalyzing the reaction of said reactants, said column containing predatory protozoa which consume anaerobic microorganisms and excrete essential nutrients consumed from said consumed anaerobic microorganisms for use by the remaining live anaerobic microorganisms;

allowing said reactants to react under the influence of said anaerobic microorganisms; and

simultaneously with said steps of introducing and allowing, restricting the presence of an essential growth nutrient within said influent solution to cause the population of said anaerobic microorganism to remain substantially constant and to prevent said anaerobic microorganisms from plugging said packed column.

9. The process according to claim 8 wherein said protozoa are identified as ATCC Culture No. 30539.

10. The process according to claim 8 wherein said support material has a hydrophilic charged surface having microorganisms attached to said hydrophilic charged surface.

11. The process according to claim 8 wherein said support material has a polymeric surface having anaerobic microorganisms attached to said polymeric surface.

12. The process according to claim 8 wherein said support material has a crosslinked polymeric surface having anaerobic microorganisms attached to said crosslinked polymeric surface.

13. The process according to claim 11 in which said influent solution comprises waste containing fermentable organic carbon, and said support material having attached microorganisms is prepared by the method comprising the steps of:

forming a mixture of rumen fluid, sewage sludge, and a polyelectrolyte;

contacting a support material with said mixture to cause organisms present in said mixture to attach to said support material; and

incubating microorganisms on said support in an environment comprising waste containing fermentable organic carbon.

14. The process according to claim 13 in which said waste comprises municipal sewage.