Short sample detection

• US 4,130,394 A
• Filed: 10/03/1977
• Issued: 12/19/1978
• Est. Priority Date: 10/03/1977
• Status: Expired due to Term

• Current Assignee: Technicon Instruments Corporation
• Original Assignee: Technicon Instruments Corporation
• Inventors: Negersmith, Kent M.
• Primary Examiner(s): Serwin, R.E.
• Application Number:
  US05/839,146
• Time in Patent Office: 442 Days
• Field of Search: 23/230 R, 23/253 R, 23/259, 73/425.4 R, 73/425.6
• US Class Current: 436/179
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 2001/382 using pistons of different sections
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: Y10T 436/25625 Dilution

Abstract

A dilutor/dispenser system wherein a marker, e.g., an air bubble, is introduced at the probe inlet prior to each aspiration cycle, so as to be displaced along the probe system during such cycle. The location of the marker is detected at the end of the aspiration cycle, to indicate whether an appropriate liquid volume has been aspirated. The aspirated liquid, along with the marker and a predetermined volume of diluent, is subsequently dispensed. An alarm may be activated, if an improper volume has been aspirated.

Claims

1. A system comprising a probe system, means for introducing a marker at an inlet of said probe system, means for aspirating a predetermined volume of liquid into said probe system, said marker being displaceable along said probe system upon aspiration of liquid therein, said probe system being calibrated such that the displacement of said marker is indicative of the volume of liquid aspirated therein, and means for dispensing said aspirated liquid.

2. A system as defined in claim 1, wherein said marker is an air bubble.

3. A system as defined in claim 2, wherein said introducing means comprises means for increasing the volume of said probe system prior to aspiration of said liquid.

4. A system as defined in claim 3, wherein a portion of said probe system is formed of a resilient material, and said introducing means includes means for increasing the volume of said portion prior to aspiration of said sample.

5. A system as defined in claim 3, wherein a portion of the probe system is formed of a resilient material, and said introducing means comprises means for compressing and releasing said resilient portion, whereby an air bubble is aspirated at said probe inlet prior to aspiration of said liquid.

6. A system as defined in claim 1, further comprising means for immersing said probe inlet into liquid contained in a first receptacle to aspirate liquid therefrom and for dispensing said aspirated liquid into a second receptacle.

7. A system as defined in claim 6, wherein said probe system normally contains a diluent, said dispensing means being operative to dispense said aspirated liquid along with a predetermined volume of said diluent into said second receptacle.

8. A system as defined in claim 6, wherein said second receptacle contains a reagent for reacting with said dispensed liquid.

9. A system as defined in claim 8, wherein said reagent is lyophilized.

10. A system as defined in claim 9, further including means for operating said introducing means prior to immersion of said probe system inlet into each of said first receptacles.

11. A system as defined in claim 1, wherein said probe system includes a transparent portion disposed at a predetermined distance from said probe system inlet, said marker being displaceable to said transparent portion upon aspiration of predetermined liquid volume.

12. A system as defined in claim 1, further comprising means for supporting a plurality of first receptacles containing liquid to be aspirated and a plurality of second receptacles for dispensing liquid, and means for relatively positioning said probe system inlet with respect to alternate ones of said first and second receptacles, said positioning means including means to immerse said probe system inlet into successive ones of said first receptacles.

13. A system as defined in claim 12, further including means to cleanse the exterior portions of said probe system inlet subsequent to immersion into each of said first receptacles.

14. Apparatus as defined in claim 1, further includes means for detecting the location of said marker along said probe system.

15. A system as defined in claim 14, wherein said marker detecting means includes means for actuating an alarm.

16. An apparatus as defined in claim 14, wherein said detecting means includes a first means for optically detecting said marker at said transparent portion.

17. A system as defined in claim 16, wherein said means for optically detecting said marker comprises a light source and a first light sensing detector disposed adjacent said light source and said transparent portion.

18. A system as described in claim 17, wherein said detecting means further includes a second detector for detecting the presence of air at said probe inlet following aspiration of said liquid volume.

19. A system as defined in claim 18, further including logic means operatively connected to and responsive to said first and second detectors, and means operatively connected to and responsive to said logic means for actuating an alarm.

20. A method for determining the aspiration of a predetermined volume of liquid into a dilutor/dispenser system, said dilutor/dispenser system including a probe system, said method comprising the steps of:

filling said probe system with a diluent to be dispensed along with a predetermined volume of liquid,

introducing a marker at the inlet of said probe system,

aspirating a volume of said liquid to displace said marker along said probe system,

detecting the location of said marker along said probe system to ascertain the volume of said liquid which has been aspirated, and

dispensing said aspirated volume of said liquid along with said marker and a predetermined volume of said diluent in accordance with said marker location.