Blood analyzer system

• US 4,135,883 A
• Filed: 08/29/1977
• Issued: 01/23/1979
• Est. Priority Date: 08/29/1977
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim Corporation (Roche Holding AG)
• Original Assignee: Bio-Dynamics, Inc. (Dun & Bradstreet Corp.)
• Inventors: Stahl, Robert M., Goldstein, Robert H., Piepho, Sandra L., McNeil, Mark D.
• Primary Examiner(s): Serwin, R.E.
• Application Number:
  US05/828,522
• Time in Patent Office: 512 Days
• Field of Search: 23/253 R, 23/259, 23/230 R, 233/26
• US Class Current: 422/72
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 2035/00495 Centrifuges
• CPC Class Codes: G01N 2035/00772 mechanical or optical code other than bar code
• CPC Class Codes: G01N 2035/0449 using centrifugal transport of liquid
• CPC Class Codes: G01N 2035/1032 Dilution or aliquotting
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/1002 Reagent dispensers

Abstract

Medical testing apparatus including a microprocessor controlled centrifuge having a group of cuvettes therein. The apparatus also includes a microdiluter apparatus with a pair of pumps therein of differing diluting capability. The cuvettes have hollow main bodies produced from transparent material to allow analysis of the specimen within the cuvettes by a series of spectrophotometers adapted to read the cuvettes while they are rotating within the centrifuge. Bags of reagent are received within the cuvettes and are designed to burst upon reaching a given level of centrifugal force allowing the reagent to flow from the bags into a test chamber within the cuvette main body. Projections are provided on the cuvettes to control the orientation of the cuvettes when inserted into the microdiluter apparatus. A tab is provided on each cuvette in proper orientation to actuate the appropriate microdiluter pump for the test the cuvette is intended for. The cuvette projections also are coded with a binary code to produce a signal which indicates to the test apparatus which test is being carried out in the cuvette. The apparatus also includes means for receiving the signal from the code reader and from the spectrophotometer and for printing out the test results appropriate for the coding.

Claims

1. Apparatus for performing chemical tests comprising:

(a) a centrifuge having a rotor, said rotor including cuvette-retaining means;

(b) a cuvette having a test chamber portion therein and test information coding thereon, said cuvette being mountable into said cuvette-retaining means;

(c) a spectrophotometer mounted on said centrifuge and adapted to read the test chamber portion of said cuvette;

(d) means for reading the test information coding on said cuvette, said code reading means being mounted on said centrifuge;

(e) and means for receiving the signal from said code reading means and from said spectrophotometer and for displaying the test result appropriate for said test information coding.

2. The apparatus of claim 1 additionally comprising:

a microdiluter including two pump sections of different dilution capability, each having an aspirator dispenser hose;

said microdiluter having a receptacle therein which is shaped to permit insertion of said cuvette only when the cuvette is in a given orientation relative to said receptacle;

a pair of switches mounted on said microdiluter, one associated with one of said pump sections and the other associated with the other of said pump sections;

each of said switches being positioned to be actuated by tab on the cuvette with the tab being oriented in accordance with the desired dilution of the liquid substance to be inserted into the cuvette.

3. The apparatus of claim 2 wherein:

said cuvette is shaped for insertion into said receptacle only when the cuvette is in a given orientation relative to said receptacle;

a tab mounted on said cuvette and oriented to actuate one or the other of said switches in accordance with the desired dilution of the liquid substance to be inserted into the cuvette.

4. The apparatus of claim 3 wherein said cuvette comprises:

a hollow main body adapted to receive a liquid substance, said main body including at least two opposed end walls to allow testing of said liquid substance by a spectrophotometer while said liquid substance is within said main body;

a first bag of reagent positioned within said main body, said bag having bursting means operable to allow said reagent to escape said bag and mix with said liquid substance upon exposing said main body to a predetermined amount of centrifugal force;

a lid sealingly mounted on said hollow main body.

5. The apparatus of claim 4 and further comprising:

desiccant means mounted in said hollow main body being operable to absorb vapor within said hollow main body prior to said vapor being absorbed by said bag of reagent.

6. The apparatus of claim 5 wherein:

said bag of reagent includes an outer main body of plastic sheet of a predetermined permeability;

said desiccant means includes a container of desiccant attached to said lid, said container includes an outer wall of plastic of a permeability higher than said predetermined permeability of said bag of reagent.

7. The apparatus of claim 4 wherein:

said main body includes a flange extending outwardly therefrom allowing the insertion of said cuvette into said receptacle only when said main body is in a given orientation relative to said receptacle, said flange being adapted to engage said rotor for locking said cuvette in said rotor, said test information coding being on said flange.

8. The apparatus of claim 4 wherein:

said lid has said tab mounted thereon extending outwardly of said main body, said lid being mounted to said main body to position said tab in a different orientation relative to said main body depending on the dilution and quantity of said liquid substance to be inserted into said main body.

9. The apparatus of claim 8 wherein:

said lid includes an aperture extending therethrough; and

further comprising;

a removable plug mounted on said lid and sealing said aperture.

10. The apparatus of claim 9 wherein said cuvette has a further flange extending oppositely of said first mentioned flange, said rotor having hooking members thereon adapted to engage said flanges to hold said cuvette on said rotor.

11. Apparatus for performing chemical tests comprising:

(a) a centrifuge having a rotor with a plurality of cuvette mounting stations thereon;

(b) at least one reagent containing prepackaged cuvette having a test chamber portion therein and test information coding thereon and mounted on the rotor of said centrifuge at one of said stations;

said test information coding corresponding to the test to be run in said cuvette;

(c) a spectrophotometer mounted on said centrifuge and adapted to read the test chamber portion of said cuvette;

(d) means for reading the test information coding on said cuvette, said code reading means being mounted on said centrifuge;

(e) and means for receiving a signal from said code reading means and a signal from said spectrophotometer and for displaying the test result appropriate for said test information coding.

12. The apparatus of claim 11 wherein said rotor has a plurality of holes therein around the rotor with each hole located at a respective one of the various stations, a further hole located just adjacent to one of said plurality of holes, means for detecting and counting said holes as the rotor rotates for counting the positions, means for resetting the counting means actuated by the further hole, said counting means producing a synch pulse which is coupled to said code reading means for the strobing of information therefrom whereby the test result displayed is associated with the cuvette rotor position as well as the test information coding on the cuvette.

13. The apparatus of claim 11 wherein said means for receiving a signal from said code reading means and a signal from said spectrophotometer comprises a microprocessor, bags received within said cuvette and containing the reagents of said cuvettes, said bags being adapted to burst in response to a high rotary speed of said centrifuge rotor, said microprocessor having a memory which is programmed to move said rotor through a cycle which includes, in order, a back and forth mixing motion of the rotor, a low speed rotary motion of the rotor for reading the specimen in the test chamber portion without reagents, a high speed rotary motion for bag breaking and causing the reagents within the bags to flow into the test chamber portion, and a low speed rotary motion of the rotor for reading test results.

14. The apparatus of claim 13 wherein said memory has a random access portion wherein is stored required constants, acquired data and calculated data.

15. The apparatus of claim 11 wherein said means for receiving a signal from said code reading means and a signal from said spectrophotometer comprises a microprocessor, said apparatus including further spectrophotometers mounted on said centrifuge at various locations and adapted to read the test chamber of said cuvette, each of said spectrophotometers including filters of differing wave lengths for measuring the cuvette light passage at differing wave lengths, said microprocessor being arranged to reject all but one of the signals from the respective spectrophotometers as determined by the test information coding on said cuvette.

16. The apparatus of claim 11 wherein said means for receiving a signal from said code reading means and a signal from said spectrophotometer and for displaying comprises a microprocessor controlled dot matrix printer.

17. The apparatus of claim 11 wherein said means for receiving a signal from said code reading means and a signal from said spectrophotometer comprises a microprocessor, a control panel including means for the operator to enter data, select function and mode, and start and stop the apparatus, said control panel also including instrument status display.

18. The apparatus of claim 11 additionally including a heater in said centrifuge for controlling the temperature of the cuvette mounted on said rotor, thermistor means mounted in said centrifuge adjacent said rotor and coupled to said heater for maintaining the temperature of said cuvette at approximately 37°

C.

19. Apparatus for performing chemical tests comprising:

(a) a centrifuge having a rotor with a plurality of cuvette mounting stations thereon;

(b) a plurality of reagent containing prepackaged cuvettes each having a test chamber portion therein and test information coding thereon and mounted on the rotor of said centrifuge each at a different one of said stations;

said test information coding on each of said cuvettes corresponding to the test to be run for said cuvette;

(c) a spectrophotometer mounted on said centrifuge and positioned to read the test chamber portion of each of said cuvettes;

(d) means for reading the test information coding on each cuvette as it passes a given location on the centrifuge and for simultaneously reading the station at which the cuvette is mounted, said code reading means being mounted on said centrifuge;

(e) and means for receiving a signal from said code reading means and a signal from said spectrophotometer and for displaying the test result for each station appropriate for said test information coding.

20. The apparatus of claim 19 wherein said spectrophotometer is one of a plurality of spectrophotometers, all of which are mounted on said centrifuge and adpated to read the test chamber portions of said cuvettes, said apparatus also comprising means for selecting the correct spectrophotometer readings for each station as indicated by the test information coding on the cuvette at each station for use in displaying the test result for each station.

21. The apparatus of claim 19 additionally comprising bags received within said cuvettes and containing the reagents of said cuvettes, said bags being adapted to burst in response to a high rotary speed of said centrifuge rotor, and means for causing said centrifuge to move through a cycle which includes, in order, a back and forth mixing motion of the rotor, a low speed rotary motion of the rotor for reading the specimen in the test chamber portion without reagents, a high speed rotary motion for bag breaking and causing the reagents within the bags to flow into the test chamber portion, and a low speed rotary motion of the rotor for reading test results.

22. The apparatus of claim 21 wherein said means for receiving and displaying is capable of performing kinetic and end point tests in any numbers at any of the cuvettes on said rotor.

23. The apparatus of claim 19 wherein said cuvettes each have flanges thereon, said flanges having said coding thereon and also serving as means for mounting said cuvette on said rotor.

24. Chemical test apparatus comprising:

two pump sections of different dilution capability each having an aspirator dispenser hose;

said apparatus having a receptacle therein which is shaped to permit insertion of a cuvette only when the cuvette is in a given orientation relative to said receptacle;

a pair of switches mounted on said apparatus, one associated with one of said pump sections and the other associated with the other of said pump sections;

each of said switches being positioned to be actuated by tab on the cuvette with the tab being oriented in accordance with the desired dilution of the liquid substance to be inserted into the cuvette.

25. The apparatus of claim 24 additionally comprising:

a cuvette shaped for insertion into said receptacle only when the cuvette is in a given orientation relative to said receptacle.

a tab mounted on said cuvette and oriented to actuate one or the other of said switches in accordance with the desired dilution of the liquid substance to be inserted into the cuvette.