Nucleic acid and protein binding paper

• US 4,139,346 A
• Filed: 11/28/1977
• Issued: 02/13/1979
• Est. Priority Date: 11/28/1977
• Status: Expired due to Term

• Current Assignee: Enzo Biochem Incorporated
• Original Assignee: Enzo Biochem Incorporated
• Inventors: Rabbani, Elazar S.
• Primary Examiner(s): Serwin, R.E.
• Application Number:
  US05/855,330
• Time in Patent Office: 442 Days
• Field of Search: 23/253 TP, 23/230 B, 23/230.3, 23/230.6, 424/1, 252/301.1 R
• US Class Current: 422/401
• CPC Class Codes: C08B 15/06 containing nitrogen , e.g. carbamates
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes

Abstract

A paper modified with aminobenzyloxymethyl groups for binding, upon activation, nucleic acid residues or proteins. The immobilized residues are analyzed by hybridization with isotope-labeled probes.

Claims

1. A modified cellulosic paper comprising a sheet or continuous web of cellulosic fibers substituted with meta-aminobenzyloxymethyl groups, said modified paper adapted to be activated by diazotization for covalent binding thereto of nucleic acid residues, proteins or mixtures thereof.

2. The modified paper of claim 1 substituted with diazotized meta-aminobenzyloxymethyl groups.

3. The paper of claim 1 covalently bonded with nucleic acid residues.

4. The paper of claim 3 wherein said nucleic acid residues are denatured DNA residues.

5. The paper of claim 3 wherein said nucleic acid residues are RNA residues.

6. The paper of claim 1 covalently bonded with protein chains.

7. The paper of claim 4 hybridized with isotopelabeled RNA residues.

8. The paper of claim 5 hybridized with isotopelabeled DNA residues.

9. A process for preparing a modified cellulosic paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a sheet or continuous web of cellulosic fibers with 1-[(meta-nitrobenzyloxy) methyl] pyridinium chloride and thereafter recovering a meta-nitrobenzyloxymethyl substituted sheet or web, then reducing said nitrobenzyloxymethyl substituents to aminobenzyloxymethyl substituents and thereafter recovering said modified cellulosic paper.

10. The process of claim 9 including diazotizing the amino moieties of said aminobenzyloxymethyl substituents to form a diazotized paper and thereafter contacting said diazotized paper with a nucleic acid residue or a protein chaine to immobilize said chain or residue.

11. The process of claim 9 wherein said nucleic acid residue is a DNA or RNA residue.

12. The process of claim 11 wherein said DNA or RNA residue is hybridized to a corresponding isotope labeled RNA or DNA probe residue to detect the size and identity of the immobilized residue.

13. A modified cellulose paper substituted with diazotizable functional groups for covalently binding nucleic acid residues, proteins or mixtures thereof.

14. A process for preparing a modified cellulose paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a cellulosic paper with a reagent to form covalently bonded substituents having a meta-nitro substituted aryl group, reducing the nitro groups to amino groups and recovering the modified paper.