Nucleic acid and protein binding paper
First Claim
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1. A modified cellulosic paper comprising a sheet or continuous web of cellulosic fibers substituted with meta-aminobenzyloxymethyl groups, said modified paper adapted to be activated by diazotization for covalent binding thereto of nucleic acid residues, proteins or mixtures thereof.
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Abstract
A paper modified with aminobenzyloxymethyl groups for binding, upon activation, nucleic acid residues or proteins. The immobilized residues are analyzed by hybridization with isotope-labeled probes.
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14 Claims
- 1. A modified cellulosic paper comprising a sheet or continuous web of cellulosic fibers substituted with meta-aminobenzyloxymethyl groups, said modified paper adapted to be activated by diazotization for covalent binding thereto of nucleic acid residues, proteins or mixtures thereof.
- 9. A process for preparing a modified cellulosic paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a sheet or continuous web of cellulosic fibers with 1-[(meta-nitrobenzyloxy) methyl] pyridinium chloride and thereafter recovering a meta-nitrobenzyloxymethyl substituted sheet or web, then reducing said nitrobenzyloxymethyl substituents to aminobenzyloxymethyl substituents and thereafter recovering said modified cellulosic paper.
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13. A modified cellulose paper substituted with diazotizable functional groups for covalently binding nucleic acid residues, proteins or mixtures thereof.
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14. A process for preparing a modified cellulose paper adapted for activation by diazotization and subsequent covalent bonding thereto by nucleic acid residues, proteins or mixtures thereof comprising treating a cellulosic paper with a reagent to form covalently bonded substituents having a meta-nitro substituted aryl group, reducing the nitro groups to amino groups and recovering the modified paper.
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