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Ultrapure hyaluronic acid and the use thereof

  • US 4,141,973 A
  • Filed: 10/25/1977
  • Issued: 02/27/1979
  • Est. Priority Date: 10/17/1975
  • Status: Expired due to Term
First Claim
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1. A sterile, pyrogen-free, protein-free, non-antigenic, hyaluronic acid fraction having an average molecular weight of at least about 750,000, a protein content of less than 0.5% by weight, ultraviolet light absorbance of a 1% solution of the sodium salt thereof of less than 3.0 at 257 nanometers wavelength and less than 2.0 at 280 nanometers wavelength, a kinematic viscosity of a 1% solution of the sodium salt thereof in physiological buffer of greater than about 1000 centistokes and a molar optical rotation of a 0.1-0.2% solution of the sodium salt thereof in physiological buffer of less than -11 = 103 degree -- cm2 /mole (of disaccharide) measured at 220 nonometers;

  • and which is characterized by infiltration by no more than about 200 white blood cells per mm3 of aqueous humor of the owl monkey eye when one milliliter of a 1% solution of the sodium salt of said fraction dissolved in physiological buffer is implanted in the vitreous replacing about one-half the existing liquid vitreous.

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