Method and reagent for the assay of hydroperoxide
First Claim
1. In a process for the photometric determination of hydroperoxides per se in a protein-containing body fluid, or indirectly of substances therein which react to liberate hydroperoxides, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μ
- M, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
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Abstract
In a method for detection and/or assay of hydroperoxides (or, indirectly, of substances, such as cholesterol or glucose, which react to liberate hydroperoxides) using an assay reagent comprising a water-soluble iodide, a redox catalyst and a stabilizer, an improvement is provided wherein when the determination is made in the presence of protein, the redox catalyst concentration in the reagent is 5-500 μM and the stabilizer concentration in the reagent is 5-100 mg/l. A corresponding improvement in the assay reagent is also provided.
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Citations
9 Claims
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1. In a process for the photometric determination of hydroperoxides per se in a protein-containing body fluid, or indirectly of substances therein which react to liberate hydroperoxides, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μ
- M, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
- View Dependent Claims (2, 3, 4)
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5. In a process for the photometric determination in a protein-containing body fluid of hydrogen peroxide liberated from cholesterol treated with cholesterol oxidase, using an assay reagent comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of the redox catalyst in the reagent is 5-500 μ
- M, and the concentration of stabilizer is 5-100 mg/l whereby the body fluid does not require deproteinization before the photometric determination.
- View Dependent Claims (6)
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7. In a reagent for the photometric determination of hydroperoxides per se or indirectly of substances which react to liberate hydroperoxides, comprising a water-soluble iodide, a redox catalyst and stabilizer, the improvement wherein the concentration of redox catalyst is 5-500 μ
- M and the concentration of stabilizer is 5-100 mg/l.
- View Dependent Claims (8)
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9. A method of detecting and assaying hydroperoxide in the presence of protein which comprises photometrically measuring the amount of triiodide ion formed when a reagent comprising water-soluble iodide, a redox catalyst in a concentration of 5-500 μ
- M and stabilizer in a concentration of 5-100 mg/l is mixed with a protein- and hydroperoxide-containing test specimen.
Specification