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Specific binding - adsorbent assay method and test means

  • US 4,145,406 A
  • Filed: 04/11/1977
  • Issued: 03/20/1979
  • Est. Priority Date: 04/11/1977
  • Status: Expired due to Term
First Claim
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1. A method for determining a specific binding substance in a liquid sample, comprising the steps of:

  • (a) contacting said sample with (1) a specific binding partner of the substance to to determined, which binding partner is incorporated with a label and which labeled binding partner has a pI value different from that of said substance to be determined, and (2) a solid ion exchange adsorbent (i) of an anionic type when the pI value of said substance to be determined is less than that of said binding partner, or (ii) of a cationic type when the pI value of said substance to be determined is greater than that of said labeled binding partner; and

    (b) determining the amount of the label which either (i) becomes associated with the adsorbent by binding of the labeled binding partner with adsorbed substance to be determined or (ii) remains unassociated therefrom,wherein step (a) is performed under conditions of pH between the pI value of the substance to be determined and that of said labeled binding partner.

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