Affinity detection apparatus

• US 4,146,365 A
• Filed: 11/07/1977
• Issued: 03/27/1979
• Est. Priority Date: 11/07/1977
• Status: Expired due to Term

• Current Assignee: Litton Bionetics, Inc.
• Original Assignee: Litton Bionetics, Inc.
• Inventors: Kay, John W. D., Kirkegaard, Leslie H.
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US05/849,154
• Time in Patent Office: 505 Days
• Field of Search: 23/230 B, 23/259, 424/12
• US Class Current: 422/402
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

In an affinity detection apparatus wherein the specific recognition of a first chemical for a second chemical is used to identify the presence of either, and being of the type normally including a receptical tray having at least one well sized to hold both an insertable member and a solution containing the first of said chemicals, and

An insertable member coated with the second chemical, the improvement comprising:

The coated member and well being complimentary shaped so as to define a first thin passageway between the member and well along substantially all the well periphery.

Claims

1. In an affinity detection apparatus wherein the specific recognition of a first chemical for a second chemical is used to identify the presence of either, and wherein said apparatus includes a receptical tray having at least one well, said well sized to hold both an insertable member and a solution containing the first of said chemicals, and

an insertable member coated with the second chemical, the improvement comprising:

the surface area of the coated member and the well wall being complimentary shaped so as to define a first thin passageway between the insertable member and the well along substantially all the well wall.

2. The apparatus of claim 1 wherein the insertable member includes a cavity communicating with at least one external surface thereof, the walls of the cavity being coated with the second chemical.

3. The apparatus of claim 2 wherein at least a portion of the well is defined between the outer periphery of an inner wall and the inner periphery of an outer wall so as to have a generally ring-shaped cross-section, and

the member includes an interior, generally longitudinal bore in communication with at least one end face and sized to circumvent the inner wall of the well upon the insertion of the endface into the well, whereby a second fluid-accomodating passageway is defined between the outer periphery of the inner well wall and the bore wall.

4. The apparatus of claim 3 wherein the inner well wall is defined by the lateral periphery of a generally upwardly projecting protuberance extending from the bottom of the well.

5. The apparatus of claim 4 wherein the well has a generally annular cross-section in the direction transverse to the well depth, and

the insertable member is a generally cylindrical shell of a thickness sized to freely fit within the annular well.

6. The apparatus of claim 4 wherein the insertable member includes an interior region of magnetically attractable material.

7. The apparatus of claim 6 further including a cover member adapted for placement on the receptical tray and having a magnetic region overlying the well during such placement for magnetically extracting the member therefrom and wherein the upwardly facing end of the inserted member is complimentary to the surface of the cover member overlying the well.

8. The apparatus of claim 6 wherein the insertable member includes a magnetically attracted core which is non-toxic to the solution and an immunochemical-attractive overcoating.

9. The apparatus of claim 8 wherein the overcoating is formed from polystyrene.

10. The apparatus of claim 8 wherein the magnetic core includes an interior magnetic portion and protective coating.

11. The apparatus of claim 10 wherein the core coating is formed from cadmium.

12. An affinity detection apparatus wherein the specific recognition of a first chemical for a second chemical is used to identify the presence of either, and wherein said apparatus includes a receptical tray having a matrix of wells sized to hold both an insertable member and a solution containing the first of said chemicals, the improvement comprising:

an insertable member coated with the second chemical, and having a bore, the insertable member and the well being complimentary shaped so as to orient the bore in a generally vertical direction in communication with the upper region of the well and to define a first thin passageway between the insertable member and well along substantially all the well wall; and

a cover member adapted for placement on the receptical tray and having a matrix of protuberances spaced to overlie the wells during such placement and sized to fit with the bores of the insertable members to maintain the insertable members in alignment with the wells during simultaneous transfer of the members into and out of the tray.