Test means and method for detecting ketone bodies

• US 4,147,514 A
• Filed: 11/21/1977
• Issued: 04/03/1979
• Est. Priority Date: 11/21/1977
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Tabb, David L., Magers, Thomas A.
• Primary Examiner(s): Wolk, Morris O.
• Assistant Examiner(s): Turk, Arnold
• Application Number:
  US05/853,390
• Time in Patent Office: 498 Days
• Field of Search: 23/230 B, 23/253 TP, 252/408
• US Class Current: 436/128
• CPC Class Codes: G01N 33/64 involving ketones
• CPC Class Codes: Y10T 436/200833 Carbonyl, ether, aldehyde or ketone containing

Abstract

Test means, method of making a test device and process for detection of ketone bodies are disclosed. More particularly, suitable test means are of the type having a nitroprusside in combination with at least one inorganic salt of a metal selected from the group of magnesium and calcium. Optionally, at least one primary amine is combined therewith. The compositions are advantageously incorporated with a carrier, such as a matrix or tablet, to provide a test device. The metal salt stabilizes the nitroprusside in solution at alkaline pH, allowing for a single-dip production method, promotes ionization of ketone bodies, resulting in a shortened reaction time, and stabilizes the resulting chromophoric complex. The chromophoric complex is further stabilized by the primary amine.

Claims

1. A test composition for detection of ketones at alkaline pH which comprises a soluble nitroprusside in combination with a compound to produce said alkaline pH and at least one inorganic salt of a metal selected from the group consisting of magnesium and calcium.

2. The test composition of claim 1 wherein the metal salt is selected from the group consisting of MgSO₄ and CaCl₂.

3. The test composition of claim 1 wherein there is present a combination of metal salts.

4. The test composition of claim 3 wherein said combination comprises MgSO₄ and CaCl₂.

5. The test composition of claim 1 wherein said nitroprusside is sodium nitroprusside.

6. A ketone detection device which comprises a carrier matrix and, incorporated therewith, the test composition of claim 1.

7. The device of claim 6 in which the carrier matrix is bibulous.

8. A ketone detection device which comprises a tablet incorporated with the test composition of claim 1.

10. A process for the preparation of a ketone test device which comprises incorporating a carrier matrix with the test composition of claim 1.

11. The process of claim 10 wherein said incorporating is impregnating with a solution of said test composition, followed by drying.

12. A process for detection of ketone in a liquid sample which comprises contacting said sample with the test composition of claim 1 and observing any resultant color formed.

13. A process for detection of ketone in a liquid sample which comprises contacting said sample with the device of claim 6 and observing any resultant color formed.

9. A ketone detection device which comprises a carrier matrix incorporated with a composition comprising sodium nitroprusside, MgSO₄ and a component to produce an alkaline pH.

14. A test composition for the detection of ketones at an alkaline pH which comprises a soluble nitroprusside, a component to produce said alkaline pH, at least one inorganic salt of a metal selected from the group consisting of magnesium and calcium and at least one primary amine.

15. The test composition of claim 14 wherein the at least one metal salt is selected from the group consisting of MgSO₄ and CaCl₂.

16. The test composition of claim 14 wherein the at least one metal salt is MgSO₄.

17. The test composition of claim 14 wherein the at least one metal salt comprises the combination of MgSO₄ and CaCl₂.

18. The test composition of claim 14 wherein the primary amine is selected from those having a pKa approximately the same as the pH at which the test means is to be used.

19. The test composition of claim 14 wherein the primary amine is aminomethanesulfonic acid, sulfanilic acid, cysteic acid, cyclohexamine, pyridoxamine, ethylene diamine, picolylamine, m-aminophenol or semicarbazide.

20. The test composition of claim 19 wherein the primary amine is aminomethanesulfonic acid.

21. The test composition of claim 14 wherein the nitroprusside is sodium nitroprusside.

22. A ketone detection device which comprises a carrier matrix and, incorporated therewith, the test composition of claim 14.

23. A process for the preparation of a ketone test device which comprises incorporating a carrier matrix with the test composition of claim 14.

24. A process for detection of ketone in a liquid sample which comprises contacting said sample with the test composition of claim 14 and observing any resultant color formed.

25. A process for detection of ketone in a liquid sample which comprises contacting said sample with the device of claim 22 and observing any resultant color formed.