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Method and apparatus for background correction in photoluminescent analysis

  • US 4,150,295 A
  • Filed: 01/05/1978
  • Issued: 04/17/1979
  • Est. Priority Date: 01/05/1978
  • Status: Expired due to Term
First Claim
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1. A method of photoluminescent background correction in the detection of a photoluminescent target substance admidst photoluminescent ambient substances comprising,selecting a photoluminescent target substance from the group consisting of fluorophores and substances labelled with fluorophores, said fluorophores having a sharp photoluminescent emission spectrum in comparison with the combined broad emission spectrum of ambient substances,sampling and measuring an amount of photoluminescence in a first selected narrow wavelength band centered at the peak of the principal emission wavelength of the selected target substance, said amount of photoluminescence composed of both target and ambient photoluminescence, said photoluminescence derived from an amount of excitation energy,sampling and measuring an amount of ambient photoluminescence in at least one other narrow wavelength band proximate to but substantially outside of said first selected band, said photoluminescence derived from said amount of excitation energy,converting said measured amount of ambient photoluminescence in the other selected wavelength bands into an amount which would occur from ambient photoluminescence measured in said first selected wavelength band, andsubtracting said converted amount from the measured amount due to both target and ambient substances.

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