Crystalline potassium salt of .beta.-nicotinamide adenine dinucleotide phosphoric acid and method for its preparation

• US 4,151,349 A
• Filed: 09/23/1977
• Issued: 04/24/1979
• Est. Priority Date: 02/01/1977
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Brustmann, Herbert, Traeger, Heinrich, Haid, Erich
• Primary Examiner(s): Brown, Johnnie R.
• Assistant Examiner(s): Hazel, Blondel
• Application Number:
  US05/836,112
• Time in Patent Office: 578 Days
• Field of Search: 536/27, 536/28
• US Class Current: 536/26.24
• CPC Class Codes: C07H 19/207 the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide

Abstract

The invention provides a novel crystalline potassium salt of β-nicotinamide adenine dinucleotide phosphoric acid (β-NADP-K), having a primitively orthorhombic crystal system, which salt is more pure and more stable than the conventional amorphous form, and is advantageously not hygroscopic. The novel salt is produced by treating a dilute aqueous solution of the potassium salt of β-nicotinamide adenine dinucleotide phosphoric acid with a hydrophilic water soluble organic solvent at a pH value of 2 to 5 until turbidity sets in, permitting crystallization and then separating the crystals.

Claims

1. Crystalline potassium salt of β

-nicotinamide adenine dinucleotide phosphoric acid in a primitively orthorhombic crystal system, with the space group P2₁ 2₁ 2₁ or P2₁ 2₁ 2, and the cell dimensions a=52.23 A±

0.03, b=16.89 A±

0.03, c=8.45 A±

0.03.