Multi-zone analytical element and method using same
First Claim
1. In a multi-zone analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous liquid, said element comprising(1) a spreading zone to receive and distribute therewithin an applied sample of said aqueous liquid, and(2) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to form a detectable species within said element,the improvement wherein said spreading zone comprises an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
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Abstract
A multi-zone analytical element for the analysis of a proteinaceous or protein-bound substance contained in an aqueous liquid and a method of using the same are disclosed. The multi-zone element contains a spreading zone and a reagent zone in fluid contact. The reagent zone of the element contains an interactive composition. Incorporated in the spreading zone of the element is an immobilized polymeric material containing charge-bearing functional groups that, under conditions of use of the element, exhibit an electric charge opposite to that of said proteinaceous substance or the protein portion of said protein-bound substance.
46 Citations
15 Claims
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1. In a multi-zone analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous liquid, said element comprising
(1) a spreading zone to receive and distribute therewithin an applied sample of said aqueous liquid, and (2) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to form a detectable species within said element, the improvement wherein said spreading zone comprises an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
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11. An integral multilayer analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous fluid, said element comprising a radiation-transmissive support bearing a reagent layer and, superposed thereover, an isotropically porous spreading layer,
(a) said reagent layer being in fluid contact with said spreading layer under conditions of use of said element and comprising a composition interactive with said analyte to provide a radiometrically detectable change within said element, and (b) said spreading layer comprising an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
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12. A method for the analysis of a proteinaceous or protein-bound analyte in an aqueous liquid sample, said method comprising
(1) contacting said aqueous liquid sample together with a multi-zone analytical element having (a) a spreading zone to receive and distribute therewithin said sample and (b) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to provide a detectable change in said element, said spreading zone containing an immobile polymeric material which (i) is substantially non-interfering with respect to said interactive composition and (ii) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte; - and
(2) detecting, after a predetermined time, the detectable change produced in said element.
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Specification