Method for determining the fibrinogen content of physiological liquid

• US 4,154,656 A
• Filed: 03/21/1977
• Issued: 05/15/1979
• Est. Priority Date: 04/10/1976
• Status: Expired due to Term

• Current Assignee: Knoll AG Chemische Fabriken
• Original Assignee: Knoll AG Chemische Fabriken
• Inventors: Maurer, Robert
• Primary Examiner(s): Wiseman, Thomas G.
• Application Number:
  US05/779,584
• Time in Patent Office: 785 Days
• Field of Search: 195/62, 195/99, 195/103.5 R, 195/66 B, 195/66 R, 195/63, 195/68, 424/94, 424/98
• US Class Current: 435/35
• CPC Class Codes: C12N 9/64 derived from animal tissue
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12Q 1/56 involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
• CPC Class Codes: G01N 2333/75 Fibrin; Fibrinogen
• CPC Class Codes: G01N 2400/22 Dextran

Abstract

What is disclosed is an enzyme system cleaving fibrinogen, said system comprising a fibrinogenase and a water-soluble polysaccharide.

Claims

1. The method for determining the fibrinogen content of a physiological liquid, which comprises mixing one part of the physiological liquid with about one part of a solution of a fibrinogenase found in viper venom and a water-soluble dextran in a quantity to enhance the enzymatic activity said solution containing one to ten units of enzyme per milliliter, permitting turbidity to develop in the resulting mixture, and comparing the turbidity developed in the resulting mixture with that of a reference standard.