Microtest plates

• US 4,154,795 A
• Filed: 07/21/1977
• Issued: 05/15/1979
• Est. Priority Date: 07/23/1976
• Status: Expired

• Current Assignee: Dynatech Holdings, Ltd.
• Original Assignee: Dynatech Holdings, Ltd.
• Inventors: Thorne, Anthony C.
• Primary Examiner(s): Scovronek, Joseph
• Application Number:
  US05/817,748
• Time in Patent Office: 663 Days
• Field of Search: 23/292, 23/259 US, 195/127, 195/140, 206/460, 206/558, 206/562, 220/23.2, 220/23.4, 220/23.6, 220/23.8, 211/74 US
• US Class Current: 422/553
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells
• CPC Class Codes: G01N 31/20 using microanalysis, e.g. drop reaction
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543

Abstract

A microtest plate is characterized by a tray conveniently moulded of foam plastics, having a multiplicity of compartments and a multiplicity of individual wells fitted into the compartments, at least some of the wells preferably being moulded integrally with one another, and preferably being arranged in one or more rows.

Claims

1. In a microtitration plate having a multiplicity of wells for containing microliter quantities of fluid samples for analysis and arranged on the plate in straight rows at right angles relative to one another, the improvement comprising means for enabling the wells to be individually removable from the plate, said means comprising a tray formed as a one piece plastics moulding, a multiplicity of discrete wells, at least some of which are moulded integrally with one another and are interconnected by stem means whereby said at least some of said wells are readily separable from one another, and means on said tray defining a multiplicity of compartments arranged in straight rows at right angles relative to one another, said discrete wells being disposed in upright positions in said compartments, and said means defining said compartments including means engaging the side walls of said wells whereby to retain said wells in said upright positions.

2. A microtitration plate as claimed in claim 1, wherein said stem means interconnect said at least some of said wells in at least one straight row to form at least one strip of wells.

3. A microtitration plate as claimed in claim 1, wherein said tray has a bottom and means defining apertures extending through said bottom substantially centrally of said compartments whereby to enable optical readings to be taken of fluid samples in said wells.

4. A microtitration plate as claimed in claim 1, wherein said means engaging said side walls of said wells comprises rows and columns of posts upstanding from said tray.

5. A microtitration plate as claimed in claim 1, wherein said tray has a peripheral wall having an upper surface and said wells have tops which project beyond said upper surface of said peripheral wall to facilitate individual removal of said wells from said tray.

6. A microtitration plate as claimed in claim 1, wherein at least some of said wells are sealed by an adhesive cover strip to seal at least a strip of wells.

7. A microtitration plate as claimed in claim 6, wherein said cover strip defines perforations therein which extend around the individual wells.

8. A microtitration plate as claimed in claim 1, wherein at least some of said wells are sealed by plugs.

9. A microtitration plate as claimed in claim 8, wherein said plugs are carried by a cover strip to seal at least a strip of wells, the cover strip defining perforations around the plugs.

10. A microtitration plate as claimed in claim 1, wherein said tray has means defining a rebate therein and said plate further comprises a lid which fits into the rebate and clears the tops of said wells.

11. A microtitration plate as claimed in claim 1, wherein:

(a) the tray comprises a bottom and means defining a rectangular wall extending around the periphery of the bottom and defining a rectangular recess within the said wall;

(b) said compartment defining means divides said recess into said multiplicity of compartments;

(c) said means engaging said side walls of said wells includes a multiplicity of discrete posts projecting upwardly from the tray bottom and disposed at equally spaced positions in rows extending at right angles relative to one another;

(d) said posts projecting upwardly from the tray bottom by a distance less than the height of said peripheral wall;

(e) posts moulded integrally with said peripheral wall at each end of each of said rows;

(f) each compartment is defined by four adjacent posts disposed at the corners of a square zone;

(g) means define apertures extending through the tray bottom substantially centrally of said compartments; and

(h) said discrete wells are moulded from plastics material and one of said wells is supported in each compartment with the bottom of the wells seated on the tray bottom.