Process for preparing a biological composition for use as a reference control in diagnostic analysis
First Claim
1. A process for making a biological composition for use as a blood serum reference composition in diagnostic analysis comprising:
- (a) adding calcium to citrated plasma and mixing well to form decitrated plasma;
(b) adding thrombin to said decitrated plasma and mixing well to form a clot of fibrin;
(c) performing at least one freeze-thaw routine on said clot to cause the fibrin to contract;
(d) removing the fibrin to thereby obtain serum;
(e) molecular washing and ultrafiltering the serum and thereby obtaining concentrated serum; and
(f) adding to the concentrated serum at least one alkylene polyol having from 2-5 carbon atoms in an amount such that said composition'"'"'s non-biological component comprises from about 60 to about 80 weight percent water and from about 20 to about 40 weight percent of said alkylene polyol.
0 Assignments
0 Petitions
Accused Products
Abstract
A process for making a biological composition for use as a blood serum reference composition in diagnostic analysis. The process comprises:
(a) adding calcium to citrated plasma and mixing well to form decitrated plasma;
(b) adding thrombin to the decitrated plasma and mixing well to form a clot of fibrin;
(c) performing at least one freeze-thaw routine on the clot to cause the fibrin to contract;
(d) removing the fibrin to thereby obtain serum;
(e) molecular washing and ultrafiltering the serum and thereby obtaining concentrated serum; and
(f) adding to the concentrated serum at least one alkylene polyol having from 2-5 carbon atoms in an amount such that said composition'"'"'s non-biological component comprises from about 40 to about 85 weight percent water and from about 15 to about 60 weight percent of said alkylene polyol.
-
Citations
10 Claims
-
1. A process for making a biological composition for use as a blood serum reference composition in diagnostic analysis comprising:
-
(a) adding calcium to citrated plasma and mixing well to form decitrated plasma; (b) adding thrombin to said decitrated plasma and mixing well to form a clot of fibrin; (c) performing at least one freeze-thaw routine on said clot to cause the fibrin to contract; (d) removing the fibrin to thereby obtain serum; (e) molecular washing and ultrafiltering the serum and thereby obtaining concentrated serum; and (f) adding to the concentrated serum at least one alkylene polyol having from 2-5 carbon atoms in an amount such that said composition'"'"'s non-biological component comprises from about 60 to about 80 weight percent water and from about 20 to about 40 weight percent of said alkylene polyol. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
Specification