Composition, test device and method for determining the presence of urobilinogen in a test sample

• US 4,158,546 A
• Filed: 07/24/1978
• Issued: 06/19/1979
• Est. Priority Date: 07/24/1978
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Rupe, Chauncey O., Lam, Charles T. W.
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US05/927,517
• Time in Patent Office: 330 Days
• Field of Search: 23/230 B, 23/253 TP, 252/408
• US Class Current: 436/97
• CPC Class Codes: G01N 33/72 involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
• CPC Class Codes: Y10T 436/146666 Bile pigment

Abstract

A novel test composition, device and method for determining the presence of urobilinogen in a test sample. The composition is capable of producing a detectable response, such as a change in color or change in light absorption, thus indicating the presence of urobilinogen, both on a qualitative and a semi-quantitative basis. Briefly, the composition comprises para-di(lower alkyl)aminobenzaldehyde as an indicator compound, a buffer substance capable of producing a pH of about 0.5 to 3, and a compound having the structure ##STR1## in which X is O or S, and in which R and R'"'"', same or different, are lower alkyl, or R and R'"'"' together are lower alkylene or ##STR2## wherein n is an integer of 1 to about 6. The device comprises a carrier matrix incorporated with the composition. The method of use comprises contacting a test sample with the composition or device and observing any detectable response formed therein.

Claims

1. In a composition capable of producing a detectable response in the presence of urobilinogen in a test sample, which composition comprises p-di(lowr alkyl)aminobenzaldehyde as an indicator and a buffer substance capable of producing a pH of about 0.5 to 3, the improvement wherein said composition further comprises a compound having the structure ##STR7## in which X is O or S;

and in which R and R'"'"', same or different, are lower alkyl, or in which R and R'"'"' together are lower alkylene or ##STR8## wherein n is an integer from 1 to about 6.

2. The improvement of claim 1 in which R and R'"'"' together form --CH₂)_n, in which n is an integer of 1 to about 6.

3. The improvement of claim 1 in which said compound is 2-imidazolidone.

4. The improvement of claim 3 in which said indicator compound is p-dimethylaminobenzaldehyde.

5. The improvement of claim 1 in which said compound is malonyl urea.

6. The improvement of claim 5 in which said indicator is p-diethylaminobenzaldehyde.

9. A device for determining the presence of urobilinogen in a test sample comprising carrier matrix incorporated with the test composition of any of claims 1-8.

10. A method for determining the presence of urobilinogen in a test sample, said method comprising contacting said sample with the composition of any of claims 1-8 and observing any detectable response.

11. A method for determining the presence of urobilinogen in a test sample, said method comprising contacting said sample with the device of claim 9 and observing any detectable response.

12. A method for preparing a test device for determining the presence of urobilinogen in a test sample comprising incorporating a carrier matrix with the composition of any of claims 1-8.

7. A composition for determining the presence of urobilinogen in a test sample, the composition comprising 2-imidazolidone, p-dimethylaminobenzaldehyde, sulfosalicylic acid, triethanolamine borate, caffeine, and ascorbic acid.

8. A composition for determining the presence of urobilinogen in a test sample, the composition comprising malonyl urea, p-diethylaminobenzaldehyde, sulfosalicylic acid, caffeine, triethanolamine borate and ascorbic acid.

13. A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, the method comprising the steps of

preparing a solution by dissolving in a suitable solvent p-di(lower alkyl)aminobenzaldehyde as an indicator, a buffer substance capable of providing a pH of about 0.5 to 3, and a compound having the structure ##STR9## in which X is O or S;

and in which R and R'"'"', same or different, are lower alkyl, or in which R and R'"'"' together are lower alkylene or ##STR10## in which n is an integer from 1 to about 6;

contacting a carrier matrix with said solution; and

drying said carrier matrix.

14. The method of claim 13 wherein said compound is one in which R and R'"'"' together form

15. The method of claim 13 wherein said compound is 2-imidazolidone.

16. The method of claim 13, 14, or 15 wherein said indicator is p-dimethylaminobenzaldehyde.

17. The method of claim 13 wherein said compound is malonyl urea.

18. The method of claim 17 wherein said indicator is p-diethylaminobenzaldehyde.

19. A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, said method comprising the steps of

preparing an impregnating solution by dissolving in a suitable solvent, sulfosalicylic acid, p-dimethylaminobenzaldehyde, triethanolamine borate, 2-imidazolidone, caffeine and ascorbic acid;

impregnating a carrier matrix with said solution by immersing said matrix in said solution; and

then, after removing it from said solution,

drying said carrier matrix.

20. A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, said method comprising the sequential steps of

preparing a solution by dissolving in a suitable solvent sulfosalicylic acid, caffeine, ascorbic acid, triethanolamine borate, p-diethylaminobenzaldehyde, and malonyl urea;

impregnating a carrier matrix with said solution by immersing said matrix in said solution; and

drying said carrier matrix.