Flying spot fluoro-meter for oxidized flavoprotein and reduced pyridine nucleotide
First Claim
1. A method of fluorometrically measuring the metabolic condition of an area of animal tissue comprising irradiating the area with a spot of excitation radiation, deflecting the spot to form a repeated geometric scanning raster pattern on the area and causing emission of radiation from points on which the spot of excitation radiation is incident whose intensity is in accordance with the metabolic state of such points, generating respective electrical signals whose amplitudes are in accordance with the intensities of radiation emitted from said points, and forming a display from said signals synchronized with said raster pattern and showing the relative intensities of the radiation from said points.
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Abstract
A method and apparatus for measuring the heterogeneity of oxygen delivery to perfused and in situ organs. A laser is employed as a flying spot scanning excitation fluorometer source which provides excitation for either oxidized flavoprotein of the mitochondrial space or reduced nucleotide of mitochondrial and cytosolic spaces. Emission from the two fluorochromes is acquired at 104 to 105 data points per sec. and histograms of the fluorescence intensity versus the number of occurrences of that intensity are displayed. The histograms show alterations of the intensity and the degree of heterogeneity of the redox states of the organ tissue under study.
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Citations
18 Claims
- 1. A method of fluorometrically measuring the metabolic condition of an area of animal tissue comprising irradiating the area with a spot of excitation radiation, deflecting the spot to form a repeated geometric scanning raster pattern on the area and causing emission of radiation from points on which the spot of excitation radiation is incident whose intensity is in accordance with the metabolic state of such points, generating respective electrical signals whose amplitudes are in accordance with the intensities of radiation emitted from said points, and forming a display from said signals synchronized with said raster pattern and showing the relative intensities of the radiation from said points.
- 9. A fluorometer apparatus for measuring the metabolic state of an area of animal tissue comprising a source of excitation radiation, means to direct said radiation from said source in the form of a beam to said area, means to deflect the beam to provide a repeated flying spot raster pattern on said area, whereby to generate emission radiation from the respective illuminated spots of the area of intensities in accordance with their metabolic state, photoelectric sensing means, means to direct the emission radiation to said sensing means, whereby to generate electrical signals in accordance with the emission intensities of the illuminated spots, and means to form an output on-line histogram display from said electrical signals indicating the relative intensities of the radiation emitted from said respective illuminated spots.
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