Reduction of detectable species migration in elements for the analysis of liquids

• US 4,166,093 A
• Filed: 06/16/1978
• Issued: 08/28/1979
• Est. Priority Date: 08/08/1977
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Smith-Lewis, Margaret J., Figueras, John
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US05/916,173
• Time in Patent Office: 438 Days
• Field of Search: 422/56, 23/230 B, 195/103.5 R, 195/103.5 C
• US Class Current: 422/421
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

An element for the analysis of liquids contains a radiation-transmissive, detectable species migration-inhibiting layer interposed between a porous radiation-blocking layer and a radiation-transmissive reagent layer. All three layers are permeable to a predetermined analyte. The reagent layer contains a composition that provides a detectable species such as a dye in proportion to the concentration of the analyte that diffuses into the reagent layer from the overlying porous radiation-blocking layer. The detectable species migration-inhibiting layer acts to reduce the migration of, for example, dye from the reagent layer into the porous radiation-blocking layer, where the optical density of the dye cannot easily be measured. Optionally, the above-described three layers can be carried on a radiation-transmissive support, and other layers such as spreading layers, registration layers, and subbing layers can also be present in the element.

Claims

1. In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a radiometrically detectable species, and a porous radiation-blocking layer permeable to said analyte;

the improvement comprising a radiation-transmissive, detectable species migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said detectable species migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said radiometrically detectable species to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.

2. An element as described in claim 1 wherein said detectable species migration-inhibiting layer comprises an immobilized antibody for said radiometrically detectable species.

3. In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and a porous radiation-blocking layer permeable to said analyte;

the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said dye migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said dye to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.

4. In an element for the analysis of liquids, said element comprising a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a radiometrically detectable species, a spreading layer permeable to said analyte, and a porous radiation-blocking layer, permeable to said analyte and interposed between said reagent layer and said spreading layer;

the improvement comprising a radiation-transmissive, detectable species migration-inhibiting layer interposed between the reagent layer and the porous radiation-blocking layer, said detectable species migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said radiometrically detectable species to said porous radiation-blocking layer upon contact of said element with the liquid under analysis.

5. In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and an outermost radiation-blocking spreading layer permeable to said analyte;

the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and inhibiting the migration of said dye to said radiation-blocking spreading layer upon contact of said element with the liquid under analysis.

6. An element as described in claim 5 which further comprises at least one radiation-transmissive registration layer interposed between said reagent layer and said support.

7. An element as described in claim 5 which further comprises a radiation-transmissive subbing layer interposed between said reagent layer and said support.

8. An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a mordant for said dye.

9. An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a mordant for the dye, said mordant having the structure ##STR5## each of R¹, R² and R³, which may be the same or different, is selected from alkyl, alkenyl, aralkyl, or aryl having less than about eight carbon atoms;

R⁴ is a ballasting group having more than about 8 carbon atoms; and

X.sup.⊖

is an acid anion.

10. An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a mordant for the dye, said mordant being polymeric and containing a recurring unit having the structure ##STR6## wherein L is a linking group between Q and the atoms in the chain of the polymer backbone;

n is 0 or 1;

X.sup.⊖

is an acid anion; and

Q.sup.⊕

is a linear or heterocyclic ammonium, phosphonium, or sulfur-containing group having one of the following structures;

##STR7## each of R¹, R², and R³, which may be the same or different, is selected from alkyl, alkenyl, aralkyl, or aryl having less than about 8 carbon atoms;

each of R⁵, R⁶, R⁷, and R⁸, which may be the same or different, represent H or R¹ as defined above; and

D is the atoms necessary to complete a heterocyclic ring.

11. An element as described in claim 5 wherein the dye migration-inhibiting layer comprises a hydrophilic colloid.

12. In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a composition that is interactive in the presence of said analyte to provide a dye, and an outermost radiation-blocking spreading layer permeable to said analyte;

the improvement comprising a radiation-transmissive, dye migration-inhibiting layer interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and comprising a hydrophilic colloid and a mordant for said dye.

13. An element as described in claim 12 wherein the radiation-blocking spreading layer comprises a blushed polymer and a pigment.

14. An element as described in claim 12 wherein the reagent layer comprises a hydrophilic colloid having said interactive composition distributed therein.

15. In an element for the analysis of liquids, said element comprising a radiation-transmissive support having thereon a radiation-transmissive reagent layer permeable to a predetermined analyte, which layer comprises a hydrophilic colloid, said colloid having distributed therein a composition that is interactive in the presence of said analyte to provide a dye;

and an outermost radiation-blocking spreading layer, permeable to said analyte, comprising a finely-divided particulate pigment and a blushed polymer;

the improvement comprising a radiation-transmissive dye migration-inhibiting layer, interposed between the reagent layer and the radiation-blocking spreading layer, said dye migration-inhibiting layer being permeable to said analyte and comprising a mordant for said dye and a hydrophilic colloid.

16. An element as described in claim 15 wherein the reagent layer is permeable to glucose and wherein the interactive composition in the reagent layer comprises glucose oxidase, peroxidase, and an indicator composition comprising a compound oxidizable in the presence of hydrogen peroxide and peroxidase to effect formation of said dye.

17. An element as described in claim 15 wherein the reagent layer is permeable to calcium and wherein the interactive composition in the reagent layer comprises an indicator for calcium.