Apparatus for measuring light intensities

• US 4,171,909 A
• Filed: 03/25/1977
• Issued: 10/23/1979
• Est. Priority Date: 03/25/1977
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Kramer, Donald L., White, James A.
• Primary Examiner(s): Corbin, John K.
• Assistant Examiner(s): Rosenberger, R. A.
• Application Number:
  US05/781,180
• Time in Patent Office: 942 Days
• Field of Search: 356/180, 356/186, 356/201, 356/206, 356/207, 356/209, 356/210, 356/212, 356/202, 356/204, 356/208, 356/72, 356/73, 356/409, 356/414, 356/432, 356/433, 356/435, 356/436 , 356/445, 356/446, 356/438
• US Class Current: 356/73
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems

Abstract

Apparatus for measuring reflected or transmitted light is described. The apparatus comprises filter type spectrophotometer having a wavelength range of from about 200 nm (nanometers) to about 1,100 nm which includes a sample illuminating light source capable of producing an extremely intense light of short duration; light diffusing means; a light filter for transmission of a selected wave-length; at least two independent light sensing means; and separate means defining light transmission paths for the passage of light from the light source to one light sensing means and from the illuminated sample to the other light sensing means.

In a preferred embodiment the apparatus includes a light diffusing integrating sphere. In another embodiment, a replaceable or repositionable module can be used to convert the apparatus from reflectance to transmittance spectrophotomer.

Claims

1. Spectrophotometer apparatus comprising:

a light source capable of providing a bright light of short duration for illumination of a sample;

sample light sensing means;

reference light sensing means;

a light reflective integrating sphere interposed between said light source and said sample and between said light source and said reference sensing means;

means establishing a first light transmission path for the passage of light from said sample to said sample light sensing means, wherein said means establishing a first light transmission path has a reflectance mode in which it directs light reflected from a sample to a sample light sensing means and wherein said means establishing a first light transmission path also has an alternate transmittance mode in which it directs light passing through a sample to said sample light sensing means;

means defining a second light transmission path for the passage of light from said light source to the reference light sensing means;

filter means in at least one of said first and second light transmission paths, said filter means permitting the transmission of light having a wavelength between about 200 and about 1100 nanometers; and

means for comparing the light received by the sample light sensing means with the light received by the reference light sensing means.

2. The apparatus of claim 1 in which the means defining said first and second light transmission paths are provided with means limiting light flow therethrough substantially to collimated rays.

3. The apparatus of claim 1 in which said light source is capable of producing a light intensity of at least 5,000°

Kelvin for a duration of from about one microsecond to about 100 microseconds.

4. The apparatus of claim 1 wherein said sample and said reference light sensing means are solid state silicon photodiodes.

5. The apparatus of claim 1 wherein said first light transmission path is positioned to direct light reflected from said sample toward said sample light sensing means.

6. The apparatus of claim 1 wherein said first light transmission path is positioned to direct light which has passed through said sample toward said sample light sensing means.

7. The apparatus of claim 1 which further comprises a transparent receptacle for accomodation of a liquid sample, and wherein said first light transmission path is positioned to direct light transmitted through said sample toward said sample light sensing means.

8. The apparatus of claim 1 in which a sample is exposed within said integrating sphere such that light reflected from said sample is transmitted along said first light transmission path.

9. The apparatus of claim 1 wherein said filter means permits transmission therethrough of light having a wavelength of between about 300 and about 725 nanometers.

10. The apparatus of claim 1 wherein said filter means permits transmission therethrough of light having a wavelength of between about 250 and about 800 nanometers.