Method for the production of a vehicle substance which is capable of covalent bonding with biologically active matter
First Claim
1. In a method for the production of a vehicle substance capable of covalent bonding with a biologically active matter which is synthetized from a vehicle containing preferably several free --NH2 or --OH groups and at least a bonding agent containing at least two reactive groups, the improvement wherein the vehicle under anhydrous conditions is reacted with an excess of organic diisocyanate in an anhydrous organic aprotic solvent in the presence of a strong basic catalyst, and the resultant vehicle substance is isolated in a storable form.
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Abstract
Process for preparing a vehicle substance capable of covalent bonding withiological material, especially enzymes, by reacting a matrix containing --OH or NH2 groups with an organic diisocyanate under anhydrous conditions. The distance between the matrix and biological material may be extended by a stepwise reaction of the matrix with an organic diisocyanate, and organic spacer containing two or more --OH and/or NH2 groups and again with an organic diisocyanate. The coupling of the vehicle material with biological material takes place in buffered aqueous solution.
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26 Claims
- 1. In a method for the production of a vehicle substance capable of covalent bonding with a biologically active matter which is synthetized from a vehicle containing preferably several free --NH2 or --OH groups and at least a bonding agent containing at least two reactive groups, the improvement wherein the vehicle under anhydrous conditions is reacted with an excess of organic diisocyanate in an anhydrous organic aprotic solvent in the presence of a strong basic catalyst, and the resultant vehicle substance is isolated in a storable form.
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