Device for staining biological materials

• US 4,180,060 A
• Filed: 08/08/1977
• Issued: 12/25/1979
• Est. Priority Date: 08/18/1972
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Kutter, Dolphe
• Primary Examiner(s): Kamm, William E.
• Application Number:
  US05/822,964
• Time in Patent Office: 869 Days
• Field of Search: 128/2 G, 128/2 F, 128/DIG. 5, 23/253 R, 23/253 TP, 23/259, 23/292, 73/425.4 P
• US Class Current: 600/573
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Abstract

Thin tubelets coated on their inner wall with a solid coating of a dyestuff or staining reagent are used to stain biological material by passing such material through the tubelet, optionally after mixing with a solvent, and allowing the biological material to react in the tubelet with the dyestuff or staining reagent.

Claims

1. Device for staining individual cells suspended in a biological fluid, consisting essentially of a thin tubelet and a solid coating of staining reagent for uniformly staining the suspended individual cells, which coating is applied to the inner wall of said tubelet and which is soluble in said fluid, whereby the uniform staining of the suspended individual cells for microscopic examination is effected upon removal of the reagent from the inner wall of said tubelet when dissolved in said fluid.

2. Device as claimed in claim 1, wherein the inner diameter of said tubelet is about 0.3 mm. to 1 mm.

3. The device according to claim 1, wherein the solid coating further contains a buffering substance.

4. The device according to claim 1, wherein the solid coating further conatins an anti-coagulant substance.

5. The device according to claim 1, wherein the solid coating further contains a stabilizing substance.