Reduction of gentisic acid interference in analytical elements

• US 4,184,923 A
• Filed: 11/03/1977
• Issued: 01/22/1980
• Est. Priority Date: 11/03/1977
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Schubert, Richard M.
• Primary Examiner(s): Jones, Raymond N.
• Assistant Examiner(s): Kepplinger, Esther M.
• Application Number:
  US05/848,255
• Time in Patent Office: 810 Days
• Field of Search: 195/127, 195/103.5 U, 195/103.5 R
• US Class Current: 435/10
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

Novel elements for the analysis of aqueous liquids which may contain gentisic acid as an interferent are described. Interference of gentisic acid with the analytical test reactions is reduced by using an organic solvent that dissolves the indicator composition but does not preferentially partition gentisic acid into itself from aqueous liquids.

Claims

1. In an element for the analysis of a predetermined analyte in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and permeable to said predetermined analyte, said carrier containing reagents comprising:

(a) an enzyme capable of catalyzing the oxidation of said predetermined analyte to a peroxide,

(b) a substance having peroxidative activity, and

(c) an indicator composition dispersed in an organic solvent and oxidizable by said peroxide in the presence of said substance having peroxidative activity to produce a detectable product;

the improvement wherein said organic solvent comprises a compound into which gentisic acid is not preferentially partitioned from the aqueous liquid.

2. In an element for the analysis of a predetermined analyte in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and permeable to said predetermined analyte, said carrier containing reagents comprising:

(a) an enzyme capable of catalyzing the oxidation of said predetermined analyte to a peroxide,

(b) a substance having peroxidative activity, and

(c) an indicator composition dispersed in an organic solvent and oxidizable by said peroxide in the presence of said substance having peroxidative activity to produce a detectable product;

the improvement wherein said organic solvent comprises a compound having the structural formula ##STR4## wherein R₁ and R₂ are substituted or unsubstituted straight-chain or branched-chain alkyl groups.

3. An element as described in claim 2 wherein the organic solvent is selected from the group consisting of 2,4-di-n-pentylphenol and 2,4-di-t-pentylphenol.

4. An element as described in claim 2 wherein the substance having peroxidative activity is peroxidase.

5. An element as described in claim 2 wherein the indicator composition is a leuco dye.

6. An element as described in claim 5 wherein the leuco dye is a triarylimidazole of the formula ##STR5## wherein R'"'"', R", and R'"'"''"'"''"'"' are each aryl or substituted aryl groups of up to 18 carbon atoms, such that at least one of R'"'"', R", and R'"'"''"'"''"'"' is an ortho or para hydroxy substituted aryl group, and at least one other R'"'"', R", and R'"'"''"'"''"'"' has an ortho or para electron donating substituent group.

7. An element as described in claim 6 wherein the leuco dye is 2(3'"'"',5'"'"'-dimethoxy-4'"'"'-hydroxyphenyl)-4,5-bis(4'"'"'-dimethylaminophenyl)imidazole.

8. An element as described in claim 2 wherein said predetermined analyte is uric acid and said enzyme is uricase.

9. An element as described in claim 2 wherein said predetermined analyte is lactic acid and said enzyme is lactate oxidase.

10. An element as described in claim 2 wherein said reagents further comprise a compound for adjusting pH.

11. An element as described in claim 2 wherein said carrier comprises a hydrophilic colloid selected from the group consisting of gelatin, a gelatin derivative, a cellulose derivative, a polysaccharide, an acrylamide, poly(vinyl alcohol, and poly(vinyl pyrrolidone).

12. An element as described in claim 11 wherein said carrier is coated on a support material.

13. An element as described in claim 11 wherein a spreading-reflecting layer is coated over said carrier.

14. An element as described in claim 13 wherein said carrier is coated on a support material.

15. An element as described in claim 14 wherein a layer comprising gelatin and a compound for adjusting pH is overcoated with a subbing layer and is interposed between said carrier and said spreading-reflecting layer.

16. An element for the analysis of uric acid in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and to uric acid, said carrier containing reagents comprising:

(a) uricase,

(b) peroxidase, and

(c) a leuco dye dispersed in an organic solvent having the structural formula ##STR6## wherein R₁ and R₂ are straight-chain or branched alkyl groups.

17. An element for the analysis of uric acid as described in claim 16 wherein the carrier is gelatin, the organic solvent is selected from the group consisting of 2,4-di-n-pentylphenol and 2,4-di-t-pentylphenol, the leuco dye is 2(3'"'"',5'"'"'-dimethoxy-4'"'"'-hydroxyphenyl)-4,5-bis(4'"'"'-dimethylaminophenyl) imidazole, and the reagents further comprise a compound for adjusting pH.

18. An element for the analysis of uric acid as described in claim 17 wherein said gelatin carrier is coated on a support material, a layer comprising gelatin and boric acid is coated on said gelatin carrier and is itself overcoated with a subbing layer, and said subbing layer is overcoated with a spreading-reflecting layer.

19. An element for the analysis of uric acid as described in claim 18 wherein said support material comprises polyethylene terephthalate, said subbing layer comprises poly-n-isopropylacrylamide, and said spreading-reflecting layer comprises cellulose acetate and titanium dioxide.

20. An element for the analysis of lactic acid in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and to lactic acid, said carrier containing reagents comprising:

(a) lactate oxidase,

(b) peroxidase, and

(c) a leuco dye dispersed in an organic solvent having the structural formula ##STR7## wherein R₁ and R₂ are straight-chain or branched alkyl groups.