Reduction of gentisic acid interference in analytical elements
First Claim
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1. In an element for the analysis of a predetermined analyte in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and permeable to said predetermined analyte, said carrier containing reagents comprising:
- (a) an enzyme capable of catalyzing the oxidation of said predetermined analyte to a peroxide,(b) a substance having peroxidative activity, and(c) an indicator composition dispersed in an organic solvent and oxidizable by said peroxide in the presence of said substance having peroxidative activity to produce a detectable product;
the improvement wherein said organic solvent comprises a compound into which gentisic acid is not preferentially partitioned from the aqueous liquid.
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Abstract
Novel elements for the analysis of aqueous liquids which may contain gentisic acid as an interferent are described. Interference of gentisic acid with the analytical test reactions is reduced by using an organic solvent that dissolves the indicator composition but does not preferentially partition gentisic acid into itself from aqueous liquids.
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Citations
20 Claims
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1. In an element for the analysis of a predetermined analyte in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and permeable to said predetermined analyte, said carrier containing reagents comprising:
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(a) an enzyme capable of catalyzing the oxidation of said predetermined analyte to a peroxide, (b) a substance having peroxidative activity, and (c) an indicator composition dispersed in an organic solvent and oxidizable by said peroxide in the presence of said substance having peroxidative activity to produce a detectable product; the improvement wherein said organic solvent comprises a compound into which gentisic acid is not preferentially partitioned from the aqueous liquid.
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2. In an element for the analysis of a predetermined analyte in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and permeable to said predetermined analyte, said carrier containing reagents comprising:
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(a) an enzyme capable of catalyzing the oxidation of said predetermined analyte to a peroxide, (b) a substance having peroxidative activity, and (c) an indicator composition dispersed in an organic solvent and oxidizable by said peroxide in the presence of said substance having peroxidative activity to produce a detectable product; the improvement wherein said organic solvent comprises a compound having the structural formula ##STR4## wherein R1 and R2 are substituted or unsubstituted straight-chain or branched-chain alkyl groups. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. An element for the analysis of uric acid in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and to uric acid, said carrier containing reagents comprising:
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(a) uricase, (b) peroxidase, and (c) a leuco dye dispersed in an organic solvent having the structural formula ##STR6## wherein R1 and R2 are straight-chain or branched alkyl groups. - View Dependent Claims (17, 18, 19)
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20. An element for the analysis of lactic acid in an aqueous liquid which may contain gentisic acid, said element comprising a carrier permeable to gentisic acid and to lactic acid, said carrier containing reagents comprising:
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(a) lactate oxidase, (b) peroxidase, and (c) a leuco dye dispersed in an organic solvent having the structural formula ##STR7## wherein R1 and R2 are straight-chain or branched alkyl groups.
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Specification