Ellipsoid-conic radiation collector and method

• US 4,189,236 A
• Filed: 03/20/1978
• Issued: 02/19/1980
• Est. Priority Date: 03/20/1978
• Status: Expired due to Term

• Current Assignee: Coulter Electronics, Inc.
• Original Assignee: Coulter Electronics, Inc.
• Inventors: Brunsting, Albert, Hogg, Walter R.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US05/888,560
• Time in Patent Office: 701 Days
• Field of Search: 356/39, 356/301, 356/317, 356/318, 356/336, 356/337, 356/338, 356/339, 356/340, 356/341, 356/342, 250/222 PC, 250/461 B, 350/294
• US Class Current: 356/317
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G02B 19/0023 at least one surface having optical power
• CPC Class Codes: G02B 19/0028 refractive and reflective surfaces, e.g. non-imaging catadioptric systems
• CPC Class Codes: G02B 19/0076 for use with a detector G02B19/009, G02B19/0095 take precedence

Abstract

Disclosed is a radiation collector apparatus and method primarily for counting and analyzing a flow of dilute particulate material, such as blood cells, sperm cells and the like, through the use of light detection. The radiation collector apparatus comprises a reflector chamber having an ellipsoidal reflector surface with a pair of elipsoidal foci defining a first focus, f₁₁, and second focus, f₁₂, and a second reflector surface with a primary focus, f₂₁, positioned at the same point as focus f₁₂, and a secondary focus, f₂₂. The second reflector surface has the configuration of one of the conic sections of revolution. In operation the radiation collector apparatus is provided with an intensifed beam of light and a stream of particulate material aligned to intersect the intensifed beam of light at focus f₁₁. Detectable light signals, after two reflections, are received in a focused beam by a photosensitive detector.

Claims

1. A radiation collector apparatus for analyzing individually isolated particulate material by illuminating the particulate material to produce a source of detectable radiation, comprising:

a reflector chamber including a first reflector surface and a second reflector surface,

said first reflector surface having a substantially ellipsoidal configuration with a first focus and a second focus defining a first symmetry axis,

said first reflector surface having said first focus disposed at the source of detectable radiation,

said second reflector surface having a primary focus and a secondary focus defining a second symmetry axis,

said second reflector surface having said primary focus disposed at said second focus of said first reflector surface,

said first symmetry axis being disposed in angled relationship with respect to said second symmetry axis,

whereby detectable radiation converged toward said second focus of said first reflector surface is reflected by said second reflector surface in an organized beam without significant stray light for further processing and analysis.

2. The radiation collector apparatus of claim 1,

said second reflector surface having a substantially conic section of revolution configuration.

3. The radiation collector apparatus of claim 2,

said second reflector surface having said secondary focus at infinity, whereby a collimated organized light beam proceeds from said second reflector surface.

4. The radiation collector apparatus of claim 3,

said second reflector surface comprises a substantially convex paraboloidal reflector surface.

5. The radiation collector apparatus of claim 3,

said second reflector surface comprises a substantially concave paraboloidal reflector surface.

6. The radiation collector apparatus of claim 2,

said second reflector surface having said secondary focus positioned in the proximity of said reflector chamber, whereby a convergent organized light beam proceeds from said second reflector surface.

7. The radiation collector apparatus of claim 6,

said second reflector surface comprises a substantially convex hyperboloidal reflector surface.

8. The radiation collector apparatus of claim 6,

said second reflector surface comprises a substantially concave ellipsoidal reflector surface.

9. The radiation collector apparatus of claim 1,

means for illuminating the particulate material with light at said first focus of said first reflector surface to produce light deviating from the path of the illuminating light.

10. The radiation collector apparatus of claim 9,

said light deviating from the path of the illuminating light comprising scattered light.

11. The radiation collector apparatus of claim 9,

said light deviating from the path of the illuminating light comprising fluorescent light.

12. The radiation collector apparatus of claim 2,

said first reflector surface comprising a first ellipsoid portion adjacent said first focus and an opposed second ellipsoid portion constituting the remainder of said first reflector surface,

said radiation ellipsoid portion defined by a segment of a first ellipsoid, said first ellipsoid having a pair of foci at said first focus and said second focus,

said second ellipsoid portion defined by a segment of a second ellipsoid, said second ellipsoid having a pair of foci at said first focus and said second focus,

said first ellipsoid having greater eccentricity than said second ellipsoid.

13. The radiation collector apparatus of claim 12,

said first ellipsoid portion and said second ellipsoid portion defining therebetween a discontinuous portion,

said second symmetry axis of said second reflector surface disposed in intersecting relationship with an exit window mounted in said discontinuous portion,

whereby the organized light beam from said second reflector surface may exit from said reflector chamber without a decrease in the solid angle of collection.

14. The radiation collector apparatus of claim 1,

said first reflector surface comprising a pair of confocal ellipsoid portions, a first ellipsoid portion and a second ellipsoid portion,

said first ellipsoid portion being positioned adjacent said first focus and said second ellipsoid portion constituting the remainder of said first reflector surface,

said first ellipsoid having greater eccentricity than said second ellipsoid.

15. The radiation collector apparatus of claim 1,

detector means positioned on said second symmetry axis to face said second reflector surface,

radiation restricting means having a radiation restricting aperture interposed between said second reflector surface and said detector means and aligned with said second symmetry axis, whereby said radiation restricting aperture filters out stray radiation.