Method and material for increasing the percentage of mammalian offspring of either sex

• US 4,191,749 A
• Filed: 10/11/1977
• Issued: 03/04/1980
• Est. Priority Date: 10/11/1977
• Status: Expired due to Term

• Current Assignee: Bernard J. Bryant
• Original Assignee: Bernard J. Bryant
• Inventors: Bryant, Bernard J.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US05/841,207
• Time in Patent Office: 875 Days
• Field of Search: 424/85, 424/88, 424/12, 424/105, 195/1.8
• US Class Current: 424/561
• CPC Class Codes: A61K 35/52 Sperm; Prostate; Seminal fluid; Leydig cells of testes
• CPC Class Codes: Y10S 424/811 involving sex selection or contraception

Abstract

Method, apparatus and material are disclosed for increasing the percentage of mammalian offspring of either sex. The method utilizes a male-specific antibody coupled to a solid-phase immunoabsorbent material to effect a separation of sex-determining spermatazoa derived from a semen suspension. The male-specific antibody selectively binds male-determining spermatazoa. The female-determining spermatazoa are not bound and are recovered directly from the male-specific antibody-coated immunoabsorbent material. The male-determining spermatazoa are recovered from the male-specific antibody-coated immunoabsorbent material after altering the condition thereof to inhibit binding. The apparatus includes a vertical surface comprising beads of immunoabsorbent material over which are distributed male-specific antibodies. The materials are two seminal fluids, one having a substantial preponderance of male-determining spermatazoa, and the other having a substantial preponderance of female-determining spermatazoa.

Claims

1. As a new composition, a conjugate of activated solid phase immunoabsorbent material having monospecific malespecific antibodies capable of binding male specific sperm distributed over the surface of said immunoabsorbent material and coupled thereto.

2. The composition of claim 1 in which said activated solid phase immunoabsorbent material is in the form of a plurality of discrete beads.

3. The composition claimed in claim 2 in which said plurality of beads comprise polysaccharide beads disposed in a column.

4. The composition of claim 1 wherein said immunoabsorbent material, has been activated to render it capable of accepting protein molecules by covalent bonding, by the treatment thereof with a cyanogen halide.

5. The composition of claim 3 wherein said polysaccharide beads have been activated to render them capable of accepting protein molecules by covalent bonding by treatment with a cyanogen halide.

6. The composition of claim 5 wherein the cyanogen halide is cyanogen bromide.

7. A process for separating male and female determining sperm from native semen which comprises:

(a) coupling monospecific male specific antibodies to a solid phase immunoabsorbent substrate to form a conjugate,

(b) loading a separation column with the monospecific male specific conjugate,

(c) passing semen containing male and female determining sperm through the column,

(d) separating out the female determining sperm from the column, and

(e) separating out the male determining sperm from the column.

8. The process of claim 7 wherein the step of separating out the male determining sperm comprises passing a composition consisting essentially of monospecific male specific antibody through the column.

9. The process of claim 7 wherein the coupling step (a) comprises:

activating the solid phase immunoabsorbent material to render it capable of accepting a protein by covalent bonding, and

adding a solution of monospecific male specific antibody thereto.

10. The process as claimed in claim 7 in which said body of semen contains the naturally-occurring ratio of male-determining spermatazoa to female-determining spermatoazoa prior to said separation of male-determining spermatazoa.

11. The process as claimed in claim 8 further comprising:

incorporating said male-determining spermatazoa into a fluid medium suitable for use in artificial insemination; and

artificially inseminating a female of the same species with said fluid medium containing said male-determining spermatazoa.

12. The process as claimed in claim 7 further comprising:

incorporating said female-determining spermatazoa into a fluid medium suitable for use in artifical insemination; and

artificially inseminating a female of the same species with said fluid medium containing said female-determining spermatazoa.

13. The method of increasing the percentage of mammalian offspring of either sex, comprising the step of selectively separating male-determining spermatazoa by contacting a body of seman with a male specific antibody capable of binding male specific sperm, said antibody being coupled to immunoabsorbent material.

14. The method as claimed in claim 13, further comprising the step of altering the immunoabsorbent material to release male-determining spermatazoa.