Hand-held pipetter

• US 4,195,526 A
• Filed: 02/09/1978
• Issued: 04/01/1980
• Est. Priority Date: 02/09/1978
• Status: Expired due to Term

• Current Assignee: CIBA Vision Corporation (Novartis Ag)
• Original Assignee: Corning Glass Works (Corning Incorporated)
• Inventors: Buck, Robert T., Amos, Lynn G.
• Primary Examiner(s): Swisher, S. Clement
• Application Number:
  US05/876,340
• Time in Patent Office: 782 Days
• Field of Search: 73/425.4 P, 73/425.6, 141/25, 222/215, 422/100
• US Class Current: 73/864.11
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/0213 Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps

Abstract

A pipetter for drawing fluid into capillary tubes and the like. A capillary tube is forced into an adapter coupled to one end of a vented, flexible tube. A compression wheel rides in a guideway adjacent the tube, and is rolled to continuously vary the point at which the tube is compressed in the manner of a peristaltic pump. Manual rolling of the wheel away from the capillary tube produces sufficient suction in the tube to cause liquid to be drawn into it. The pumping action is reversible in that rolling the wheel back toward the capillary tube will cause fluid to be expelled. Provision is made to prevent undesirable "compression set" as a result of long term static of the flexible tube during periods of non-use.

Claims

1. A pipetter for controllably drawing a volume of fluid from a supply into a capillary tube, comprising:

a housing having a first and a second end and a support surface extending therebetween;

a resilient tube disposed in said housing and extending over said support surface;

adapter means for replaceably receiving a sampling pipe to be filled with a fluid, said adapter means defining a passage for coupling the interior of the sampling pipe with the interior of said tube;

compression wheel means for compressing said tube against said support surface at a point intermediate the ends thereof, said compression wheel means being adapted to be moved with respect to said support surface by the user of the pipetter;

mounting means in said housing for guiding said compression wheel means in a direction generally parallel to said support surface;

whereby the point at which said tube is compressed may be continuously varied to create a lowered pressure in said adapter passage; and

means to preclude substantial compression of said tube at one position of said wheel, whereby said tube relaxes to its uncompressed state when said wheel is in said position.

2. A pipetter according to claim 1, wherein said mounting means and said support surface diverge near one of the ends thereof.

3. A pipetter according to claim 2, wherein said mounting means defines a generally linear path for said compression wheel means, and said support surface diverges from said path near one end thereof.

4. A pipetter according to claim 3, wherein said compression wheel means comprises a rotatable disk.

5. A pipetter according to claim 4, further including axle means extending from either side of said disk;

an elongate groove formed in said housing; and

a slider having an aperture receiving said axle means and disposed in said groove for movement therealong.

6. A pipetter according to claim 1, wherein said compression wheel is sufficiently eccentric to preclude substantial compression of said tube at one position of said wheel.

7. A pipetter for withdrawing a controlled volume of fluid from a supply into a sampling pipe, comprising:

a housing defining a generally rigid support surface, and an elongate guideway extending generally parallel thereto;

a resilient tube disposed within said housing and overlying said support surface;

means coupled to one end of said tube and adapted to receive a sampling pipe to be filled with fluid;

said means defining a passage for coupling the interior of said sampling tube with the interior of said resilient tube; and

a compression wheel having an axle means retained in said guideway and having a sufficient radius to compress said resilient tube against said support surface when said axle means is disposed at a predetermined point along said guideway, and being sufficiently eccentric to prevent said compression of said resilient tube at one position of said wheel.

8. A pipetter according to claim 7, wherein said rigid support surface diverges from said guideway near one end thereof, whereby when said compression wheel is disposed near one end of said guideway said resilient tubing is not substantially compressed.

9. A pipetter according to claim 8, further including an axle extending through said compression wheel, and a slider disposed in said guideway for receiving said axle means.

10. A pipetter according to claim 8, further including means for adjusting the relative spacing between said rigid support surface and said guideway.

11. A pipetter according to claim 8, further including a second adapter engaging the second end of said resilient tube and defining a passage for venting said resilient tube to the atmosphere.

12. A pipetter according to claim 9, wherein said slider is provided with at least two apertures disposed at different distances from said support surface for allowing said compression wheel to be selectively spaced by at least two different distances from said support surface.