Freeze-stable liquid blood control standard
First Claim
1. A freeze-stable blood control standard for the quality control of the measurement of blood pH and gases comprising a sealed receptacle containing treated erythrocytes and a gaseous head space having a volume which is at least equal to about the volume of said erythrocytes, said erythrocytes being treated by thorough washing in saline solution, mild admixing with a solution of aldehyde and saline, thorough washing in saline solution, then slow admixing with a cryoprotective agent selected from the group consisting of lower aliphatic diols and triols to a concentration of from about 20 to about 40 volume percent of said cryoprotective agent, said gaseous head space comprising from about 0% to about 15% CO2, from about 0% to about 25% O2 and the balance selected from the group consisting of N2 and inert gases and mixtures thereof.
0 Assignments
0 Petitions
Accused Products
Abstract
A freeze-stable liquid blood control standard and method is provided for the quality control of the measurement of blood pH and gases in the clinical laboratory. The blood control standard comprises a sealed receptacle containing specially treated red cells and a gaseous head space at least equal to about the volume of the red cells. The special treatment comprises thorough washing and separating the red cells from the plasma components and mild treatment with aldehyde, slow admixture with lower aliphatic diol or triol, and retention in a buffered solution. The special treatment optionally includes treating at least a portion of the red cells with carbon monoxide. The head space comprises from 0-15% CO2, 0-25% O2 and the balance N2 and/or inert gas.
-
Citations
17 Claims
- 1. A freeze-stable blood control standard for the quality control of the measurement of blood pH and gases comprising a sealed receptacle containing treated erythrocytes and a gaseous head space having a volume which is at least equal to about the volume of said erythrocytes, said erythrocytes being treated by thorough washing in saline solution, mild admixing with a solution of aldehyde and saline, thorough washing in saline solution, then slow admixing with a cryoprotective agent selected from the group consisting of lower aliphatic diols and triols to a concentration of from about 20 to about 40 volume percent of said cryoprotective agent, said gaseous head space comprising from about 0% to about 15% CO2, from about 0% to about 25% O2 and the balance selected from the group consisting of N2 and inert gases and mixtures thereof.
- 16. The method of making a freeze-stable blood control standard for the quality control of the measurement of blood pH and gases comprising thoroughly washing erythrocytes in saline solution, mildly admixing with a solution of aldehyde and saline, thoroughly washing in saline solution, then slowly admixing with a cryoprotective agent selected from the group consisting of lower aliphatic diols and triols to a concentration of from about 20 to about 40 volume percent of said cryoprotective agent, transferring to receptacles while leaving a gaseous head space of at least about equal to the volume of said erythrocytes, flushing said head space with a gas comprising from about 0% to about 15% CO2, from about 0% to about 25% O2 and the balance selected from the group consisting of N2 and inert gases and mixtures thereof, and immediately thereafter sealing said receptacles to form a gas tight closure.
Specification