Tissue processing method

• US 4,199,558 A
• Filed: 04/03/1978
• Issued: 04/22/1980
• Est. Priority Date: 04/07/1976
• Status: Expired due to Term

• Current Assignee: Shandon Southern Products Limited (PHC Holdings Corporation)
• Original Assignee: Shandon Southern Products Limited (PHC Holdings Corporation)
• Inventors: Henderson, Kenneth J.
• Primary Examiner(s): Kaplan, Morris
• Application Number:
  US05/892,769
• Time in Patent Office: 750 Days
• Field of Search: 427/2, 427/4, 427/248, 424/3, 23/230 B, 118/48, 118/49, 118/429, 134/11, 134/21, 134/31, 34/22, 34/26, 34/11, 8/94.11
• US Class Current: 435/40.52
• CPC Class Codes: A01N 1/00 Preservation of bodies of humans or animals, or parts thereof
• CPC Class Codes: A01N 3/00 Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions preservation or chemical ripening of fruit or vegetables A23B7/00; Grafting wax
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 2001/315 Basket-type carriers for tissues

Abstract

Histological and like tissue specimens are processed by suspending them successively in the condensing vapors of a dehydrating fluid and a clarifying fluid and permeating the tissue specimens with the respective condensed fluid.

Claims

1. A method of processing a tissue specimen, comprising the steps of suspending the specimen in condensing vapours of a volatile dehydrating agent, condensing the vapours on the specimen, and permitting the condensed vapours of the dehydrating agent to penetrate throughout the specimen and to be absorbed thereby until the moisture therein has been completely displaced.

2. The method of claim 1, wherein the dehydrated specimen is suspended in condensing vapours of a clearing agent and the condensed vapours of the clearing agent are permitted to penetrate through the dehydrated specimen until the dehydrating agent has been displaced.

3. The method of claim 2, wherein the specimen is successively suspended in the vapours of the dehydrating agent and of the clearing agent boiling at the same temperature to produce the respective vapours.

4. The method of claim 1, comprising the further step of adjusting the atmospheric pressure wherein the specimen is suspended to cause the agent to vapourize at a selected temperature.