Radioimmunoassay apparatus

• US 4,200,613 A
• Filed: 04/14/1978
• Issued: 04/29/1980
• Est. Priority Date: 06/03/1977
• Status: Expired due to Term

• Current Assignee: Ramco Laboratories Incorporated
• Original Assignee: Ramco Laboratories Incorporated
• Inventors: Alfrey, Clarence P., Bedford, Donald B.
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US05/896,176
• Time in Patent Office: 746 Days
• Field of Search: 23/259, 23/253 R, 23/230 B, 23/292, 195/127, 424/1, 424/12
• US Class Current: 422/71
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: Y10S 436/801 Electron dense compounds, e.g. ferritin
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A coated bead, a gripper for rotatably engaging the bead, a carrier for carrying at least one gripper, test tray bearing at least one reaction chamber and an agitator for agitating the carrier in relation to the test tray are disclosed.

Claims

1. Apparatus for performing a quantitative radioimmunoassay comprising:

a bead for carrying a dry antibody;

a gripper for gripping said bead, said gripper comprising

an elongate handle portion, and

a clasp portion;

a carrier for carrying said gripper, said carrier comprising

a base portion

a plurality of spaced apart collars carried by said base portion and for receiving therethrough said elongate handle portion; and

a reaction chamber for receiving said bead when said bead is gripped by said gripper and having support means to support said carrier when said gripper is carried by said carrier.

2. The apparatus of claim 1 wherein said clasp portion comprises a finger, said finger having an interior surface which is contoured to conform to a surface of said bead.

3. The apparatus of claim 2 wherein said finger further comprises an end having a camming surface to deflect said finger away from said bead when said gripper is urged towards said bead.

4. The apparatus of claim 1 wherein said bead is rotatably held by said gripper.

5. The apparatus of claim 1 wherein said apparatus comprises a plurality of said reaction chambers, said chambers being arranged in a matrix.

6. The apparatus of claim 5 wherein said collars are equidistantly spaced and linearly aligned on said base portion.

7. The apparatus of claim 6 wherein said collars are axially aligned with a row of said reaction chambers.

8. The apparatus of claim 1 and further including agitation means for agitating said carrier relative to said reaction chamber.

9. The apparatus of claim 1 wherein said clasp portion comprises a finger, said finger having a wiping edge, said bead being rotatably held by said gripper, said wiping edge being operable to wipe the surface of said bead when said bead is rotated.

10. The apparatus of claim 9 and further including agitation means for agitating said carrier relative to said reaction chamber.

11. Apparatus for performing a quantitative radioimmunoassay comprising:

a bead for carrying a dry antibody; and

a gripper for gripping said bead, said gripper comprising

an elongate handle portion, and

a clasp portion.

12. The apparatus of claim 11 wherein said clasp portion comprises a finger, said finger having an interior surface which is contoured to conform to a surface of said bead.

13. The apparatus of claim 12 wherein said finger further comprises an end having a camming surface to deflect said finger away from said bead when said gripper is urged towards said bead.

14. The apparatus of claim 11 wherein said bead is rotatably held by said gripper.

15. The apparatus of claim 11 wherein said clasp portion comprises a finger, said finger having a wiping edge, said bead being rotatably held by said gripper, said wiping edge being operable to wipe the surface of said bead when said bead is rotated.