Parabolic cell analyzer

• US 4,200,802 A
• Filed: 03/28/1979
• Issued: 04/29/1980
• Est. Priority Date: 03/28/1979
• Status: Expired due to Term

• Current Assignee: United States Department of Energy
• Original Assignee: United States Department of Energy
• Inventors: Salzman, Gary C., Skogen Hagenson, Mary J.
• Primary Examiner(s): Willis, Davis L.
• Application Number:
  US06/024,762
• Time in Patent Office: 398 Days
• Field of Search: 250/461 B, 250/461 R, 250/458, 250/372, 250/343, 250/345, 250/495, 356/317, 356/318
• US Class Current: 250/461.2
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream

Abstract

The disclosure is directed to a cell analysis apparatus incorporating a paraboloidal cavity for maximum utilization for improved cell characteristic monitoring.

Claims

1. A cell analysis apparatus comprising:

a housing containing a paraboloidal cavity having an axis of rotation, a focus, and light reflecting walls;

means for passing cells to be analyzed through said focus of said paraboloidal cavity;

means for directing a beam of essentially parallel light rays through said focus so as to impinge upon any cells passing therethrough; and

means for selectively collecting light reflected by cells passing through said beam at said focus to determine therefrom preselected characteristics of said cells.

2. The invention of claim 1 wherein substantially all said reflected light is collected to provide a total scatter signal.

3. The invention of claim 1 wherein said selective light collecting means comprises means for collecting only light reflected from said cavity walls which exits said cavity parallel to its axis of rotation.

4. The invention of claim 3 wherein said collecting means comprises a spatial filter.

5. The invention of claim 3 wherein said collecting means comprises a photodetector ring array.

6. The invention of claim 5 wherein said array comprises about sixty detectors dispersed about every 5.6 degrees.

7. A cell analysis apparatus comprising:

a housing containing a paraboloidal cavity having an axis of rotation, a focus, and light reflecting walls;

means for passing cells to be analyzed substantially through said focus of said paraboloidal cavity;

means for directing a beam of essentially parallel light of a selected wavelength through said focus to impinge upon any cell passing therethrough and to cause said cell to fluoresce; and

means for selectively collecting light fluoresced by said cells passing through said beam at said focus to determine therefrom certain cellular characteristics.

8. The invention of claim 7 wherein said collecting means comprises a spatial filter for passing only fluoresced light travelling parallel to said axis of rotation, a diffraction grating for receiving said parallel fluoresced light and for diffracting said light in at least a first order diffraction spectrum, means for focusing said first order diffraction spectrum onto a focal plane, and means for quantitatively detecting the diffraction spectrum incident on said focal plane.

9. The invention of claim 8 wherein said quantitative detecting means comprises a plurality of photomultiplier tubes.

10. A chamber structure for use in a cell analysis system comprising:

a housing containing a paraboloidal cavity having a focus and an axis of rotation;

means for passing a laser beam through said focus and essentially perpendicular to said axis of rotation; and

means comprising a nozzle protruding into said chamber structure essentially perpendicular to said axis of rotation and said laser beam for passing a stream of cells to be analyzed through said beam essentially at said focus.