Flow cell fluid and sample supply mechanism

• US 4,202,747 A
• Filed: 07/06/1978
• Issued: 05/13/1980
• Est. Priority Date: 07/06/1978
• Status: Expired due to Term

• Current Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Original Assignee: Beckman Instruments, Inc. (Danaher Corporation)
• Inventors: Meyer, Richard C., Buzza, Edmund E.
• Primary Examiner(s): Tung, T.
• Application Number:
  US05/922,458
• Time in Patent Office: 677 Days
• Field of Search: 204/1 T, 204/195 R, 422/50, 422/63, 422/98
• US Class Current: 204/411
• CPC Class Codes: G01N 33/4915 using flow cells flow cytometry G01N15/14
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

Apparatus for automatically and selectively supplying a blood sample or a wash/calibration solution to the entrance port of a flow cell which measures characteristics of the sample such as pH, pCO₂ and pO₂. A sample filled syringe and a solution supply conduit are supported by respective first and second fluid delivery mechanisms which rotate to move the syringe outlet and the conduit outlet in generally arcuate paths toward and away from the flow cell entrance port. The delivery mechanisms are rotated simultaneously, one toward and one away from the entrance port, by a common drive gear. Seating of the wash solution outlet in the entrance port is upstream of the seating of the sample outlet to ensure complete flushing of sample from the entrance port by the wash solution.

Claims

1. In a flow cell for sample analysis including a passage therein for accommodating sample flow therethrough past sample measuring apparatus, the flow cell passage having a fluid entrance port, an improved fluid and sample supply mechanism comprising:

sample supply means having an outlet through which sample is delivered;

a first delivery mechanism supporting said sample supply means for presenting the outlet thereof to the fluid entrance port of said flow cell passage;

means for rotating said first delivery mechanism about an axis of rotation generally perpendicular to the direction of flow through said fluid entrance port to move said outlet in an arcuate path toward and away from said fluid entrance port;

fluid supply means including an outlet through which fluid is delivered;

a second delivery mechanism supporting said fluid delivery means for presenting the outlet thereof to the fluid entrance port ofsaid flow cell passage;

means for rotating said second delivery mechanism about an axis of rotation generally perpendicular to the direction of flow through said fluid entrance port to move the fluid delivery outlet in an arcuate path toward and away from said fluid entrance port; and

means for controlling the rotation of said first and second delivery mechanisms to selectively position the outlet of said sample supply means or the outlet of said fluid supply means at the fluid entrance port of the flow cell passage for delivering sample or fluid, as required, through said fluid entrance port into the flow cell passage.

2. The flow cell of claim 1 wherein the control means includes drive means for simultaneously rotating said first and second delivery mechanisms one toward and one away from the fluid entrance port of said flow cell passage.

3. The flow cell of claim 2 wherein said drive means includes first and second driven gears and means for connecting said first and second delivery mechanisms to the respective gears to be rotated thereby;

and

a common drive gear meshed with both said first and second driven gears for simultaneously driving the same.

4. The flow cell of claim 1 wherein said fluid entrance port includes a seating surface area against which the respective outlets of said sample supply means and said fluid supply means are engageable in a fluidtight seating relationship when positioned at said fluid entrance port, the respective outlets being dimensioned such that the fluid supply means outlet seats at a location upstream of the corresponding location of the sample supply means outlet to insure contact by said fluid of all areas of said entrance port contactable by said sample.

5. The flow cell of claim 4 wherein said seating surface of said fluid entrance port is defined by a resilient seating member having a first cylindrical section extending coaxially along a portion of the flow cell passage and a second radially extending section having an exposed face in a plane generally perpendicular to direction of flow through the fluid entrance port;

said outlet of said sample supply means is defined by an axially tapering tip section which engages and seats within said first cylindrical section forming a first fluid tight seal therewith; and

said outlet of said fluid supply means engages and seats against the exposed face of said second radially extending section forming a second fluid tight seal therewith upstream of said first fluid tight seal.

6. The flow cell of claim 1 further including:

pumping means independent of the sample supply means and of the fluid supply means connected to the flow cell passage for pumping fluid therethrough; and

means for enabling the pumping means with the first and second delivery mechanisms at respective rotational positions in which neither the sample supply means outlet nor the fluid supply means outlet is positioned at the fluid entrance port of the flow cell passage thereby enabling flushing of the flow cell passage with air pumped therethrough by the pumping means.