Isoelectric focusing method

• US 4,204,929 A
• Filed: 04/18/1978
• Issued: 05/27/1980
• Est. Priority Date: 04/18/1978
• Status: Expired due to Term

• Current Assignee: University Patents, Inc.
• Original Assignee: University Patents, Inc.
• Inventors: Bier, Milan
• Primary Examiner(s): Prescott, Arthur C.
• Application Number:
  US05/897,261
• Time in Patent Office: 770 Days
• Field of Search: 204/180 R, 204/180 S, 204/180 P, 204/299 R, 204/301, 424/12, 23/230 B
• US Class Current: 204/518
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: G01N 27/44795 Isoelectric focusing

Abstract

The disclosure is directed to a method and apparatus for isoelectric focusing of fluids. In accordance with the disclosed method, the flow of fluids to be processed is established in a first direction. This flow of fluids is streamlined by providing a plurality of permeable microporous membranes which define generally parallel channels oriented in the first direction. An electrical potential is applied across the streamlined channels of flowing fluid, and isoelectric focusing is achieved on the fluids during the flow thereof since the membranes allow interchange of fluid constituents therebetween while providing the desired streamlining. An approximation of "plug" type flow is achieved within the streamlined channels; i.e., flow having an approximately uniform cross-sectional characteristic. In the preferred embodiment of the method of the invention, a recirculation path is established for each of the streamlined channels, such that the fluid flowing out of each channel is recirculated back to the beginning of the channel. Preferably, this is achieved by pumping the fluids in each of the recirculation paths, and also providing cooling for the fluids during the recirculation thereof. In this manner, a number of "passes" are effected to obtain the desired degree of isoelectric focusing. The cooling, which is performed during the recirculation, serves to minimize problems with dissipation of Joule heat during the isoelectric focusing of the fluids in the streamlined channels. Also, in the preferred embodiment of the invention, the first direction is downward such that the streamlined fluids flow under the influence of gravity to permit gravity equilibrium of fluid levels in the channels across the streamlining membranes.

Claims

1. A method for isoelectric focusing of fluids containing buffering components capable of establishing a stable pH gradient in an electric field, comprising the steps of:

establishing a flow of said fluids in a first direction;

streamlining the flow of said fluids in said first direction by providing a plurality of permeable membranes which define generally parallel channels oriented in said first direction; and

applying an electrical potential across the streamlined channels of flowing fluids.

2. The method as defined by claim 1 further comprising the step of establishing a recirculation path associated with each of said streamlined channels such that the fluid flowing out of each channel is recirculated back to the beginning of said channel.

3. The method as defined by claim 2 further comprising the step of pumping the fluids in each of said recirculation paths to establish continuous flow therein.

4. The method as defined by claim 3 further comprising the step of cooling said fluids during the recirculation thereof.

5. The method as defined by claim 1 wherein said first direction is downward such that said streamlined fluid flow is under the influence of gravity.

6. The method as defined by claim 2 wherein said first direction is downward such that said streamlined fluid flow is under the influence of gravity.

7. The method as defined by claim 3 wherein said first direction is downward such that said streamlined fluid flow is under the influence of gravity.

8. The method as defined by claim 4 wherein said first direction is downward such that said streamlined fluid flow is under the influence of gravity.

9. The method as defined by claim 3 further comprising the step of monitoring the fluid properties in said recirculation paths.

10. The method as defined by claim 4 further comprising the step of monitoring the fluid properties in said recirculation paths.

11. The method as defined by claim 5 further comprising the step of monitoring the fluid properties in said recirculation paths.

12. The method as defined by claim 8 further comprising the step of monitoring the fluid properties in said recirculation paths.

13. The method as defined by claim 3 further comprising the step of extracting fluids after a desired number of recirculations.

14. The method as defined by claim 4 further comprising the step of extracting fluids after a desired number of recirculations.

15. The method as defined by claim 5 further comprising the step of extracting fluids after a desired number of recirculations.

16. The method as defined by claim 8 further comprising the step of extracting fluids after a desired number of recirculations.

17. The method as defined by claim 12 further comprising the step of extracting fluids after a desired number of recirculations.

18. The method as defined by claim 2 further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

19. The method as defined by claim 3 further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

20. The method as defined by claim 4 further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

21. The method as defined by claim 5 further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

22. The method as defined by claim 8 further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

23. The method as defined by claim 1, wherein said buffering components comprise a mixture of amphoteric substances having a range of isoelectric points.

24. The method as defined by claim 2, wherein said buffering components comprise a mixture of amphoteric substances having a range of isoelectric points.

25. The method as defined by claim 3, wherein said buffering components comprise a mixture of amphoteric substances having a range of isoelectric points.

26. The method as defined by claim 4, wherein said buffering components comprise a mixture of amphoteric substances having a range of isoelectric points.

27. The method as defined by claim 23, wherein the application of said electrical potential across the streamlined channels in operative to establish a gradient of pH steps from channel to channel.

28. The method as defined by claim 24, wherein the application of said electrical potential across the streamlined channels is operative to establish a gradient of pH steps from channel to channel.

29. The method as defined by claim 25, wherein the application of said electrical potential across the streamlined channels is operative to establish a gradient of pH steps from channel to channel.

30. The method as defined by claim 26, wherein the application of said electrical potential across the streamlined channels is operative to establish a gradient of pH steps from channel to channel.

31. The method as defined by claim 23, wherein said plurality of permeable membranes define at least six channels.

32. The method as defined by claim 24, wherein said plurality of permeable membranes defined at least six channels.

33. The method as defined by claim 29, wherein said plurality of permeable membranes define at least six channels.

34. The method as defined by claim 30, wherein said plurality of permeable membranes define at least six channels.

35. The method as defined by claim 31, wherein each of said recirculation paths is adapted to hold at least ten times as much fluid as its respective channel.

36. The method as defined by claim 32, wherein each of said recirculation paths is adapted to hold at least ten times as much fluid as its respective channel.

37. The method as defined by claim 33, wherein each of said recirculation paths is adapted to hold at least ten times as much fluid as its respective channel.

38. The method as defined by claim 34, wherein each of said recirculation paths is adapted to hold at least ten times as much fluid as its respective channel.

39. The method as defined by claim 35, further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

40. The method as defined by claim 36, further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

41. The method as defined by claim 37, further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.

42. The method as defined by claim 38, further comprising the step of adding fluids to be processed to said fluids containing said buffering components after said buffering components have been focused to establish a pH gradient therein.