Hemoglobin-oxygen equilibrium curve analyzer
First Claim
1. An apparatus for obtaining a hemoglobin-oxygen equilibrium curve for a sample, comprising housing means including a chamber adapted to receive a quantity of said sample, a cup-shaped semipermeable membrane mounted in said chamber and defining externally thereof a substantially annular sample-receiving space within the chamber, means to supply a gaseous reagent to the interior of said cup-shaped membrane, means to compute the rate of gas transport through the membrane into a sample contained in said annular space, electrode means exposed to said annular space to measure the solution partial pressure of the transported gas in the sample, and means to plot the percent gas saturation in the sample against the changes in said partial pressure.
0 Assignments
0 Petitions
Accused Products
Abstract
A system for obtaining a hemoglobin-oxygen equilibrium curve for a sample of concentrated hemoglobin solution or whole blood. Gaseous oxygen and/or nitrogen is used to oxygenate or deoxygenate the sample. The exchange takes place across a gas-receiving cup-shaped semipermeable membrane in a temperature-controlled chamber containing the sample, in an annular space defined between the cup-shaped gas-receiving membrane and the chamber wall. The rate of oxygen transport into and out of the sample is continuously calculated by means of a microcomputer. Simultaneously the solution pO2 is measured by means of a Clark electrode exposed to the sample. From the oxygen transport rate calculation and the pO2 measurement, the percent O2 saturation is calculated and is plotted against the change in pO2. Oxygen can be exchanged bidirectionally with the sample across the semipermeable membrane, which is positioned concentrically in a cylindrical sample cavity. The sample solution is maintained well-stirred by a magnetic stirrer provided in the cavity.
-
Citations
15 Claims
- 1. An apparatus for obtaining a hemoglobin-oxygen equilibrium curve for a sample, comprising housing means including a chamber adapted to receive a quantity of said sample, a cup-shaped semipermeable membrane mounted in said chamber and defining externally thereof a substantially annular sample-receiving space within the chamber, means to supply a gaseous reagent to the interior of said cup-shaped membrane, means to compute the rate of gas transport through the membrane into a sample contained in said annular space, electrode means exposed to said annular space to measure the solution partial pressure of the transported gas in the sample, and means to plot the percent gas saturation in the sample against the changes in said partial pressure.
- 13. A method for obtaining a hemoglobin-oxygen equilibrium curve for a sample comprising enclosing the sample in a space with a semipermeable membrane on one side, supplying gas to the area of the membrane opposite to the sample for diffusion to the sample, directly measuring the partial pressure of the gaseous oxygen in the sample, determining the rate of oxygen transport into the sample by continuous solution of the diffusion equation, continuously calculating the percent of oxygen saturation, and plotting the percent of oxygen saturation against the changes in partial pressure of the oxygen in the sample.
Specification