Container for immunochemical and enzymatical determinations or procedures
First Claim
Patent Images
1. An elongated, hollow container suitable for use in an immunochemical or enzymatical procedure comprising(a) a lower reactive part composed of a polymeric material, said part being closed at one end and having on at least a portion of its inner surface a coating of an inert protein to which a biologically active substance is attached;
- and(b) a separable inert upper part connected to and in communication with said lower part.
0 Assignments
0 Petitions
Accused Products
Abstract
A container useful in immunochemical and enzymatical determinations or procedures.
42 Citations
36 Claims
-
1. An elongated, hollow container suitable for use in an immunochemical or enzymatical procedure comprising
(a) a lower reactive part composed of a polymeric material, said part being closed at one end and having on at least a portion of its inner surface a coating of an inert protein to which a biologically active substance is attached; - and
(b) a separable inert upper part connected to and in communication with said lower part. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- and
-
24. A process for preparing a lower reactive part, composed of a copolymer of polyethylene and acrylic acid, said part being closed at one end and having on at least a portion of its inner surface a coating of the inert protein bovine gamma globulin to which digoxin antibody is attached which comprises:
-
(a) cleaning with 0.5% by weight, based on the total volume of the solution, of an aqueous solution of sodium dodecyl sulfate the surface of a lower reactive part, composed of a copolymer of polyethylene, 92% by weight and acrylic acid, 8% by weight, said part being closed at one end and thereafter washing one or more times with water, (b) treating the lower reactive part of (a) with a sufficient amount of an aqueous solution of potassium hydroxide to convert the carboxyl groups on the surface to the salt form, washing one or more times with water and then with a phosphate buffer solution having a pH of about 6.4, (c) coating by adsorption the surface of the lower reactive part of (b) by immersing in a phosphate buffer solution containing the inert protein bovine gamma globulin under adsorbing conditions and thereafter washing with water one or more times and then washing with a phosphate buffer solution having a pH of about 7.4, (d) coupling sufficient glutaraldehyde to enable attachment of antibody to digoxin to the bovine gamma globulin coating on the reactive part of (c) by immersing said reactive part in a phosphate buffer solution having a pH of about 7.4, containing about 2% by weight based on the total volume of the solution of glutaraldehyde, thereafter washing with water one or more times and then washing with a phosphate buffer solution having a pH of 7.0, (e) coupling antibody to digoxin to the glutaraldehyde on the reactive part of (d) by immersing said reactive part in a phosphate buffer solution having a pH of 7.0, containing antibody to digoxin in a dilution of 1/325,000 to 1/335,000 and a protective agent, bovine serum albumin, to retard denaturization of said antibody under attaching conditions, washing with water one or more times and then washing with a glycine buffer solution having a pH of about 2.4 to remove any antibody not coupled to the glutaraldehyde, (f) washing one or more times with a phosphate buffer solution having a pH of about 7.4 and thereafter treating with a phosphate buffer solution having a pH of about 7.4 containing 2% by weight, based on the total volume of the solution, polyvinyl alcohol as a stabilizing agent, and (g) vacuum drying under conditions which will not substantially denature the antibody.
-
-
26. An elongated, hollow container suitable for use in an immunochemical or enzymatical procedure comprising
(a) a lower reactive part composed of a polymeric material containing carboxylate salt groups, said part being closed at one end and having attached on at least a portion of its inner surface a coating of an inert protein to which a biologically active substance, stabilized against denaturization, is attached by means of a chemical coupling agent through which said biologically active substance is covalently bound to said inert protein; - and
(b) a separable inert upper part connected to and in communication with said lower part. - View Dependent Claims (25, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
- and
Specification