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Method of coupling a protein to an epoxylated latex

  • US 4,210,723 A
  • Filed: 11/25/1977
  • Issued: 07/01/1980
  • Est. Priority Date: 07/23/1976
  • Status: Expired due to Term
First Claim
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1. A method for coupling a protein having a reactive group containing a labile hydrogen to polymer particles containing free epoxy groups in a latex wherein the polymer particles in the latex range in size from about 0.15 to 1.5 micrometers in diameter and have an inner core and an outer shell containing free epoxy groups, said inner core being formed by the polymerization or copolymerization of one or more hard monomers and optionally soft monomer to produce a latex containing polymer particles that are said inner core and said outer shell being formed by copolymerization in the presence of said inner core polymer particles one or more hard monomers and a copolymerizable ethylenically unsaturated compound having free epoxy groups and optionally soft monomer, said method comprising reacting said polymer particles containing free epoxy groups in the latex and said protein at a pH of 7.0-9.0 for a time sufficient to form a covalent bond between the reactive group of the protein and the free epoxy group of the polymer particles and removing the unreacted protein from the latex-protein conjugate.

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