Method and apparatus for the non-invasive determination of the characteristics of a segmented fluid stream

• US 4,210,809 A
• Filed: 03/16/1979
• Issued: 07/01/1980
• Est. Priority Date: 03/16/1979
• Status: Expired due to Term

• Current Assignee: Technicon Instruments Corporation (Bayer AG)
• Original Assignee: Technicon Instruments Corporation (Bayer AG)
• Inventors: Pelavin, Milton H.
• Primary Examiner(s): Willis, Davis L.
• Application Number:
  US06/021,034
• Time in Patent Office: 473 Days
• Field of Search: 250/258, 250/343, 250/344, 250/345, 250/356, 250/357, 250/373, 250/432 R, 250/435, 73/194 E, 73/194 F, 73/204, 356/246, 356/408, 356/39, 356/51, 356/436
• US Class Current: 250/343
• CPC Class Codes: G01F 1/7086 using optical detecting arrangements
• CPC Class Codes: G01F 1/74 Devices for measuring flow of a fluid or flow of a fluent solid material in suspension in another fluid
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

New and improved method and apparatus for the non-invasive detection of the respective segments and segment interfaces, and the non-invasive determination of the respective segment volumes, of a continuous segmented fluid stream flowing in a transparent conduit are disclosed and comprise means to irradiate the conduit and the flowing fluid stream with radiation energy of spectral content predetermined in accordance with the radiation energy absorbance and refraction characteristics of the fluid segments and the fluid segment-conduit interface, means to detect the radiation energy which is transmitted through the segments and conduit and provide signals indicative thereof, and signal processing means operatively associated with the detecting means and operable to process said signals and perform the stated functions in accordance therewith.

Claims

1. In apparatus for the detection of the respective segments, and the respective segment interfaces, of a continuous fluid stream which comprises alternating fluid segments flowing in an optionally transparent conduit, said fluid segments having different radiation energy absorbance and/or refraction characteristics, the improvements comprising, radiation energy source means disposed without said conduit and operable to irradiate the same and said flowing fluid stream, radiation energy detecting means disposed without said conduit and operable to detect the radiation energy from said source means which is transmitted through said conduit and said flowing fluid stream, and to output a signal indicative thereof, and means to process said output signal to indicate said fluid segments and said segment interfaces, respectively, wherein, said fluid segments comprise segments of liquids, and wherein the spectral content of said radiation energy is generally outside the radiation energy absorbance range of said liquids.

2. In apparatus as in claim 1 wherein, said fluid segments comprise a segment of a marker liquid, and wherein the spectral content of said radiation energy is generally within the radiation energy absorbance range of said marker liquid.

3. In apparatus as in claim 1 wherein, said fluid segments comprise segments of air.

4. In apparatus as in claim 1 wherein, said radiation energy is infra red energy.

5. In apparatus as in claim 4 wherein, the spectral content of said infra red energy is centered around a wavelength ranging between 800 and 1000 nm.

6. In apparatus as in claim 5 wherein, said fluid segments comprise blood serum and/or reagent segments, water segments, and air segments.

7. In apparatus for the determination of the respective liquid segment volumes of a continuous fluid stream which comprises alternating liquid and other fluid segments flowing in an optically transparent conduit, said liquid segments having different radiation energy absorbance and/or refraction characteristics than said other fluid segments, the improvements comprising, radiation energy source means disposed without said conduit and operable to irradiate the same and said flowing fluid stream at spaced locations along said conduit, radiation energy detecting means disposed without said conduit and operable to detect the radiation energy from said source means which is transmitted through said conduit and said flowing fluid stream at said spaced locations, and to output signals indicative thereof, and means to process said output signals to determine said liquid segment volumes.

8. In apparatus as in claim 7 wherein, said radiation energy source means comprise first and second radiation energy sources disposed at said spaced locations along said conduit to one side thereof, and said radiation energy detecting means comprise first and second radiation energy detectors disposed to the opposite side of said conduit at said spaced locations in optical alignment with said first and second radiation energy sources, respectively.

9. In apparatus as in claim 7 wherein, the spectral content of said radiation energy is generally outside the radiation energy absorbance range of said liquids.

10. In apparatus as in claim 9 wherein, the spectral content of said infra red energy is centered around a wavelength ranging between 800 and 1000 nm.

11. In apparatus as in claim 7 wherein, said fluid segments comprise segments of air.

12. In apparatus as in claim 7 wherein, said radiation energy is infra red energy.

13. In apparatus as in claim 7 wherein, said signal processing means comprise means to determine the time required for one of said liquid segments to flow in said conduit past one of said locations, and the time required for the leading edge of the same one of said liquid segments to flow in said conduit between locations.

14. In apparatus as in claim 13 wherein, said signal processing means further comprise means to determine the time required for the trailing edge of said one of said liquid segments to flow in said conduit between said locations.

15. In a method for the detection of the respective segments, and the respective segment interfaces, of a continuous fluid stream which comprises alternating fluid segments flowing in an optically transparent conduit, said fluid segments having different radiation energy absorbance and/or refraction characteristics and comprising liquid segments, the improvements comprising the steps of, irradiating said conduit and said flowing fluid stream with radiation energy having a spectral content which is generally outside the radiation energy absorbance range of said liquid segments, detecting the irradiating radiation energy which is transmitted through said conduit and said flowing fluid stream and providing a signal indicative thereof, and processing said signal to indicate said fluid segments and said segment interfaces, respectively.

16. In a method as in claim 15 wherein, said fluid segments comprise segment of a marker liquid, and wherein the spectral content of said radiation energy is generally within the radiation energy absorbance range of said marker liquid.

17. In a method as in claim 15 wherein, the step of irradiating said conduit and said flowing fluid stream comprises the irradiation thereof with infra red energy.

18. In a method as in claim 17 wherein, the step of irradiating said conduit and said flowing fluid stream comprises the irradiation thereof with infra red energy having a spectral content centered around a wavelength ranging between 800 and 1000 nm.

19. In a method for the determination of the respective liquid segment volumes of a continuous fluid stream which comprises alternating liquid and other fluid segments flowing in an optically transparent conduit, said liquid segments having different radiation energy absorbance and/or refraction characteristics than said other fluid segments, the improvements comprising the steps of, irradiating said conduit and said flowing fluid stream with radiation energy at spaced locations along said conduit, said radiation energy having a spectral content which is generally outside the radiation energy absorbance range of said liquid segments, detecting the irradiating radiation energy which is transmitted through said conduit and said flowing fluid stream at said spaced locations and providing signals indicative thereof, and processing said signals to determine said liquid segment volumes.

20. In a method as in claim 19 wherein, the step of irradiating said conduit and said flowing fluid stream comprises the irradiation thereof with infra red energy.

21. In a method as in claim 20 wherein, the step of irradiating said conduit and said flowing fluid stream comprises the irradiation thereof with infra red energy having a spectral content centered around a wavelength ranging between 800 and 1000 nm.

22. In a method as in claim 19 wherein, the processing of said signals comprises the determination of the time required for one of said liquid segments to flow in said conduit past one of said locations, and the time required for the leading edge of the same one of said liquid segments to flow in said conduit between said locations.

23. In a method as in claim 22 wherein, the processing of said signals further comprises the determination of the time required for the trailing edge of the same one of said liquid segments to flow in said conduit between said locations.

24. In apparatus for the detection of the segments and segment interfaces, and for the determination of the liquid segment volumes, respectively, of a continuous fluid stream which comprises alternating liquid and other fluid segments flowing in an optically transparent conduit, said liquid segments having different radiation energy absorbance and/or refraction characteristics than said other fluid segments, the improvements comprising, radiation energy source means disposed without said conduit and operable to irradiate the same and said flowing fluid stream at spaced locations along said conduit, radiation energy detecting means disposed without said conduit and operable to detect the radiation energy from said source means which is transmitted through said conduit and said flowing fluid stream at said spaced locations and to output signals indicative thereof, means to process said output signals to indicate said segments and said segment interfaces, respectively, and means to process said output signals to determine said liquid segment volumes.

25. In a method for the detection of the segments and segment interfaces, and for the determination of the liquid segment volumes, respectively, of a continuous fluid stream which comprises alternating liquid and other fluid segments flowing in an optically transparent conduit, said liquid segments having different radiation energy absorbance and/or refraction characteristics than said other fluid segments, the improvements comprising the steps of, irradiating said conduit and said flowing fluid stream with radiation energy at spaced locations along said conduit, detecting the irradiating radiation energy which is transmitted through said conduit and said flowing fluid stream at said spaced locations and providing signals indicative thereof, and processing said signals to indicate said segments and segment interfaces and to determine said liquid segment volumes.

26. In a method for determining thee volume of a discrete fluid segment flowing in a conduit in a continuous flowing stream, said fluid segment being disposed in said stream between two other fluid segments each defining an interface with said fluid segment, the improvements comprising the steps of measuring a first time period required for one of said interfaces to flow between two spaced locations along said conduit, measuring a second time period required for said fluid segment to flow between said spaced locations, and relating said first and second time periods to determine the volume of said fluid segment.