Optode arrangement

• US 4,215,940 A
• Filed: 07/19/1977
• Issued: 08/05/1980
• Est. Priority Date: 07/20/1976
• Status: Expired due to Term

• Current Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Original Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Inventors: Lubbers, Dietrich W., Opitz, Norbert
• Primary Examiner(s): Corbin, John K.
• Assistant Examiner(s): de los Reyes, B.W.
• Application Number:
  US05/817,109
• Time in Patent Office: 1,113 Days
• Field of Search: 356/196, 356/173, 356/39, 356/402, 356/41, 356/417, 356/85, 23/330 B, 23/232 R, 23/254 R, 23/, 128/2 G, 128/2 L, 128/214 B, 128/DIG. 23, 250/458
• US Class Current: 356/402
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53

Abstract

Monochromatic excitation light is passed into an indicator chamber to exc the indicator therein. The side of the indicator chamber in contact with the substance to be analyzed is closed off by a membrane which is permeable to the component of that substance whose concentration is to be determined. The side through which the radiation emitted from the indicator towards the light-measuring unit of the apparatus passes is transmissive for the emitted radiation. The direction of introduction of monochromatic excitation radiation, and the direction in which the radiation emitted from the indicator towards the light-measuring unit, include an angle relative to each other, and are preferably perpendicular. As a result, any spectral reactivity of the substance to be analyzed to the excitation radiation does not have an affect upon the light-measuring unit of the apparatus, and the latter receives light exclusively dependent upon the spectral alteration of the indicator in response to the concentration of the component whose concentration is to be ascertained.

Claims

1. An apparatus for the optical measurement of concentrations of components of a substance to be analyzed, of the type comprised of a source of monochromatic excitation radiation, an indicator chamber containing an indicator into which the monochromatic excitation radiation enters to excite the indicator, the side of the indicator chamber to be contacted by the substance to be analyzed being closed off by a membrane permeable for the component whose concentration is to be determined, the side of the indicator chamber through which radiation emitted from the indicator is to be emitted being transmissive for the emitted radiation, and a light-measuring unit operative for receiving the radiation emitted from the indicator and generating a corresponding signal indicative of the concentration of the component whose concentration is to be determined, the improvement wherein the travel path of the radiation emitted from the indicator to the light-measuring unit extends at an angle relative to the travel path of the monochromatic excitation radiation passing through the indicator chamber.

2. The apparatus defined in claim 1, wherein the two travel paths include with each other an angle of about 90°

.

3. The apparatus defined in claim 1, wherein the travel path of the monochromatic excitation radiation passing through the indicator chamber is generally parallel to said membrane.

4. The apparatus defined in claim 3, including a light-conductor element arranged to transmit the excitation energy from the source towards the interior of the indicator chamber.

5. The apparatus defined in claim 4, including an intermediate optical coupling element operative for receiving the radiation tramsmitted by the light-conductor element and directing it inwardly into the interior of the indicator chamber.

6. The apparatus defined in claim 5, wherein the indicator chamber and the optical coupling element are of circular configuration and the indicator chamber has a radius corresponding to the penetration depth of the monochromatic excitation radiation.

7. The apparatus defined in claim 5, wherein the indicator chamber and the optical coupling element are of elongated configuration and the indicator chamber has a transverse breadth corresponding to the penetration depth of the monochromatic excitation radiation.