Method of making printed reagent test devices

• US 4,216,245 A
• Filed: 07/25/1978
• Issued: 08/05/1980
• Est. Priority Date: 07/25/1978
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Johnson, Leighton C.
• Primary Examiner(s): Silverberg, Sam
• Application Number:
  US05/927,892
• Time in Patent Office: 742 Days
• Field of Search: 422/56, 422/57, 427/2
• US Class Current: 427/2.13
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence

Abstract

A test device for determining the presence of a constituent in a sample, and a method for making it are disclosed. The test device comprises reactants (e.g. reagents, enzymes, etc.) incorporated with a carrier matrix such that when the device is wetted with a test sample, the reactants and the constituent react to produce a detectable response. The reactants are positioned separately from each other on the matrix in substantially, discrete, non-contacting areas. Hence, reactants are maintained substantially separate from each other until the test device is wetted with the sample.

Claims

1. In a method for preparing a dip-and-read test device for determining the presence of a constituent in a test sample, whereby said method comprises incorporating a carrier matrix with a reagent system comprising first and second reactants, said reactants mutually interacting in the presence of said constituent to produce a detectable response, the improvement which comprises

preparing a first reactant ink containing said first reactant, and a second reactant ink containing said second reactant,

printing said first ink onto said carrier matrix in a first array of impressions, and

printing said second ink onto said carrier matrix in a second array of impressions, the impressions of said second array being printed so as to be at least partially interspersed with, but substantially not contacting, the impressions of said first array.

2. The method of claim 1 in which the inks are printed by offset.

3. The method of claim 1 in which the inks are printed by screening.

4. The method of claim 1 in which the inks are printed by gravure.

5. The method of claim 1 in which the first ink is printed in a plurality of parallel stripes, and the second ink is likewise printed in stripes mutually parallel to and positioned between the first ink stripes.

6. In a method for preparing a dip-and-read test device for determining the presence of a constituent in a test sample, whereby said method comprises incorporating a carrier matrix with a reagent system comprising first and second reactants, said reactants mutually interacting in the presence of said constituent to produce a detectable response, the improvement which comprises

preparing a first reactant ink containing said first reactant, and a second reactant ink containing said second reactant,

silk screening said first ink onto said carrier matrix in a first array of impressions, and

silk screening said second ink onto said carrier matrix in a second array of impressions, the impressions of said second array being printed so as to be at least partially interspersed with, but substantially not contacting, the impressions of said first array.

7. The method of claim 6 in which the first and second patterns comprise dots.

8. The method of claim 6 in which the first and second patterns together comprise a plurality of parallel stripes, the stripes of the first reactant ink being alternately positioned with respect to the stripes of the second reactant ink.